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Post-transcriptional control of miRNA biogenesis
MicroRNAs (miRNAs) are important regulators of gene expression that bind complementary target mRNAs and repress their expression. Precursor miRNA molecules undergo nuclear and cytoplasmic processing events, carried out by the endoribonucleases DROSHA and DICER, respectively, to produce mature miRNAs...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298569/ https://www.ncbi.nlm.nih.gov/pubmed/30333195 http://dx.doi.org/10.1261/rna.068692.118 |
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author | Michlewski, Gracjan Cáceres, Javier F. |
author_facet | Michlewski, Gracjan Cáceres, Javier F. |
author_sort | Michlewski, Gracjan |
collection | PubMed |
description | MicroRNAs (miRNAs) are important regulators of gene expression that bind complementary target mRNAs and repress their expression. Precursor miRNA molecules undergo nuclear and cytoplasmic processing events, carried out by the endoribonucleases DROSHA and DICER, respectively, to produce mature miRNAs that are loaded onto the RISC (RNA-induced silencing complex) to exert their biological function. Regulation of mature miRNA levels is critical in development, differentiation, and disease, as demonstrated by multiple levels of control during their biogenesis cascade. Here, we will focus on post-transcriptional mechanisms and will discuss the impact of cis-acting sequences in precursor miRNAs, as well as trans-acting factors that bind to these precursors and influence their processing. In particular, we will highlight the role of general RNA-binding proteins (RBPs) as factors that control the processing of specific miRNAs, revealing a complex layer of regulation in miRNA production and function. |
format | Online Article Text |
id | pubmed-6298569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-62985692019-01-01 Post-transcriptional control of miRNA biogenesis Michlewski, Gracjan Cáceres, Javier F. RNA Review MicroRNAs (miRNAs) are important regulators of gene expression that bind complementary target mRNAs and repress their expression. Precursor miRNA molecules undergo nuclear and cytoplasmic processing events, carried out by the endoribonucleases DROSHA and DICER, respectively, to produce mature miRNAs that are loaded onto the RISC (RNA-induced silencing complex) to exert their biological function. Regulation of mature miRNA levels is critical in development, differentiation, and disease, as demonstrated by multiple levels of control during their biogenesis cascade. Here, we will focus on post-transcriptional mechanisms and will discuss the impact of cis-acting sequences in precursor miRNAs, as well as trans-acting factors that bind to these precursors and influence their processing. In particular, we will highlight the role of general RNA-binding proteins (RBPs) as factors that control the processing of specific miRNAs, revealing a complex layer of regulation in miRNA production and function. Cold Spring Harbor Laboratory Press 2019-01 /pmc/articles/PMC6298569/ /pubmed/30333195 http://dx.doi.org/10.1261/rna.068692.118 Text en © 2019 Michlewski and Cáceres; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Review Michlewski, Gracjan Cáceres, Javier F. Post-transcriptional control of miRNA biogenesis |
title | Post-transcriptional control of miRNA biogenesis |
title_full | Post-transcriptional control of miRNA biogenesis |
title_fullStr | Post-transcriptional control of miRNA biogenesis |
title_full_unstemmed | Post-transcriptional control of miRNA biogenesis |
title_short | Post-transcriptional control of miRNA biogenesis |
title_sort | post-transcriptional control of mirna biogenesis |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298569/ https://www.ncbi.nlm.nih.gov/pubmed/30333195 http://dx.doi.org/10.1261/rna.068692.118 |
work_keys_str_mv | AT michlewskigracjan posttranscriptionalcontrolofmirnabiogenesis AT caceresjavierf posttranscriptionalcontrolofmirnabiogenesis |