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Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil

The bacterium Actinobacillus pleuropneumoniae is the etiological agent of Contagious Porcine Pleuropneumonia, a disease responsible for economic losses in the swine industry worldwide. A. pleuropneumoniae is capable of producing proteinaceous exotoxins responsible for inducing hemorrhagic lesions, o...

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Autores principales: dos Santos, Lucas Fernando, Costa Polveiro, Richard, Scatamburlo Moreira, Thalita, Pereira Vidigal, Pedro Marcus, Chang, Yung-Fu, Scatamburlo Moreira, Maria Aparecida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298653/
https://www.ncbi.nlm.nih.gov/pubmed/30562362
http://dx.doi.org/10.1371/journal.pone.0208789
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author dos Santos, Lucas Fernando
Costa Polveiro, Richard
Scatamburlo Moreira, Thalita
Pereira Vidigal, Pedro Marcus
Chang, Yung-Fu
Scatamburlo Moreira, Maria Aparecida
author_facet dos Santos, Lucas Fernando
Costa Polveiro, Richard
Scatamburlo Moreira, Thalita
Pereira Vidigal, Pedro Marcus
Chang, Yung-Fu
Scatamburlo Moreira, Maria Aparecida
author_sort dos Santos, Lucas Fernando
collection PubMed
description The bacterium Actinobacillus pleuropneumoniae is the etiological agent of Contagious Porcine Pleuropneumonia, a disease responsible for economic losses in the swine industry worldwide. A. pleuropneumoniae is capable of producing proteinaceous exotoxins responsible for inducing hemorrhagic lesions, one of which is ApxI. Few studies have conducted an in-depth evaluation of polymorphisms of the nucleotides that make up the ApxI toxin gene. Here we analyze the polymorphisms of the apxIA gene region of A. pleuropneumoniae serovar 5 isolated from swine in different regions in Brazil and report the results of molecular sequencing and phylogenetic analysis. Analysis of the apxIA gene in 60 isolates revealed the presence of genetic diversity and variability. The polymorphisms in the nucleotide sequences determined the grouping of the Brazilian sequences and five more sequences from the GenBank database into 14 different haplotypes, which formed three main groups and revealed the presence of mutations in the nucleotide sequences. The estimation of selection pressures suggests the occurrence of genetic variations by positive selective pressure on A. pleuropneumoniae in large groups of animals in relatively small spaces. These conditions presumably favor the horizontal dissemination of apxIA gene mutations within bacterial populations with host reservoirs. As a result, the same serovar can demonstrate different antigenic capacities due to mutations in the apxIA gene. These alterations in sequences of the apxIA gene could occur in other areas of countries with intense swine production, which could lead to differences in the pathogenicity and immunogenicity of each serovar and have implications for the clinical status or diagnosis of A. pleuropneumoniae.
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spelling pubmed-62986532018-12-28 Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil dos Santos, Lucas Fernando Costa Polveiro, Richard Scatamburlo Moreira, Thalita Pereira Vidigal, Pedro Marcus Chang, Yung-Fu Scatamburlo Moreira, Maria Aparecida PLoS One Research Article The bacterium Actinobacillus pleuropneumoniae is the etiological agent of Contagious Porcine Pleuropneumonia, a disease responsible for economic losses in the swine industry worldwide. A. pleuropneumoniae is capable of producing proteinaceous exotoxins responsible for inducing hemorrhagic lesions, one of which is ApxI. Few studies have conducted an in-depth evaluation of polymorphisms of the nucleotides that make up the ApxI toxin gene. Here we analyze the polymorphisms of the apxIA gene region of A. pleuropneumoniae serovar 5 isolated from swine in different regions in Brazil and report the results of molecular sequencing and phylogenetic analysis. Analysis of the apxIA gene in 60 isolates revealed the presence of genetic diversity and variability. The polymorphisms in the nucleotide sequences determined the grouping of the Brazilian sequences and five more sequences from the GenBank database into 14 different haplotypes, which formed three main groups and revealed the presence of mutations in the nucleotide sequences. The estimation of selection pressures suggests the occurrence of genetic variations by positive selective pressure on A. pleuropneumoniae in large groups of animals in relatively small spaces. These conditions presumably favor the horizontal dissemination of apxIA gene mutations within bacterial populations with host reservoirs. As a result, the same serovar can demonstrate different antigenic capacities due to mutations in the apxIA gene. These alterations in sequences of the apxIA gene could occur in other areas of countries with intense swine production, which could lead to differences in the pathogenicity and immunogenicity of each serovar and have implications for the clinical status or diagnosis of A. pleuropneumoniae. Public Library of Science 2018-12-18 /pmc/articles/PMC6298653/ /pubmed/30562362 http://dx.doi.org/10.1371/journal.pone.0208789 Text en © 2018 dos Santos et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
dos Santos, Lucas Fernando
Costa Polveiro, Richard
Scatamburlo Moreira, Thalita
Pereira Vidigal, Pedro Marcus
Chang, Yung-Fu
Scatamburlo Moreira, Maria Aparecida
Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil
title Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil
title_full Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil
title_fullStr Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil
title_full_unstemmed Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil
title_short Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil
title_sort polymorphism analysis of the apxia gene of actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298653/
https://www.ncbi.nlm.nih.gov/pubmed/30562362
http://dx.doi.org/10.1371/journal.pone.0208789
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