Cargando…

Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy

Painful diabetic neuropathy (PDN) is a common and troublesome diabetes complication. Protein kinase C (PKC)-mediated dorsal root ganglia (DRG) P2X3 receptor upregulation is one important mechanism underlying PDN. Accumulating evidence demonstrated that electroacupuncture (EA) at low frequency could...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Ya-feng, Ying, Xiao-ming, He, Xiao-fen, Shou, Sheng-Yun, Wei, Jun-Jun, Tai, Zhao-xia, Shao, Xiao-mei, Liang, Yi, Fang, Fang, Fang, Jian-qiao, Jiang, Yong-liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298917/
https://www.ncbi.nlm.nih.gov/pubmed/30084084
http://dx.doi.org/10.1007/s11302-018-9617-4
_version_ 1783381379834183680
author Zhou, Ya-feng
Ying, Xiao-ming
He, Xiao-fen
Shou, Sheng-Yun
Wei, Jun-Jun
Tai, Zhao-xia
Shao, Xiao-mei
Liang, Yi
Fang, Fang
Fang, Jian-qiao
Jiang, Yong-liang
author_facet Zhou, Ya-feng
Ying, Xiao-ming
He, Xiao-fen
Shou, Sheng-Yun
Wei, Jun-Jun
Tai, Zhao-xia
Shao, Xiao-mei
Liang, Yi
Fang, Fang
Fang, Jian-qiao
Jiang, Yong-liang
author_sort Zhou, Ya-feng
collection PubMed
description Painful diabetic neuropathy (PDN) is a common and troublesome diabetes complication. Protein kinase C (PKC)-mediated dorsal root ganglia (DRG) P2X3 receptor upregulation is one important mechanism underlying PDN. Accumulating evidence demonstrated that electroacupuncture (EA) at low frequency could effectively attenuate neuropathic pain. Our previous study showed that 2-Hz EA could relieve pain well in PDN. The study aimed to investigate whether 2-Hz EA relieves pain in PDN through suppressing PKC-mediated DRG P2X3 receptor upregulation. A 7-week feeding of high-fat and high-sugar diet plus a single injection of streptozotocin (STZ) in a dose of 35 mg/kg after a 5-week feeding of the diet successfully induced type 2 PDN in rats as revealed by the elevated body weight, fasting blood glucose, fasting insulin and insulin resistance, and the reduced paw withdrawal threshold (PWT), as well as the destructive ultrastructural change of sciatic nerve. DRG plasma membrane P2X3 receptor level and DRG PKC expression were elevated. Two-hertz EA failed to improve peripheral neuropathy; however, it reduced PWT, DRG plasma membrane P2X3 receptor level, and DRG PKC expression in PDN rats. Intraperitoneal administration of P2X3 receptor agonist αβ-meATP or PKC activator phorbol 12-myristate 13-acetate (PMA) blocked 2-Hz EA analgesia. Furthermore, PMA administration increased DRG plasma membrane P2X3 receptor level in PDN rats subject to 2-Hz EA treatment. These findings together indicated that the analgesic effect of EA in PDN is mediated by suppressing PKC-dependent membrane P2X3 upregulation in DRG. EA at low frequency is a valuable approach for PDN control.
format Online
Article
Text
id pubmed-6298917
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-62989172018-12-31 Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy Zhou, Ya-feng Ying, Xiao-ming He, Xiao-fen Shou, Sheng-Yun Wei, Jun-Jun Tai, Zhao-xia Shao, Xiao-mei Liang, Yi Fang, Fang Fang, Jian-qiao Jiang, Yong-liang Purinergic Signal Original Article Painful diabetic neuropathy (PDN) is a common and troublesome diabetes complication. Protein kinase C (PKC)-mediated dorsal root ganglia (DRG) P2X3 receptor upregulation is one important mechanism underlying PDN. Accumulating evidence demonstrated that electroacupuncture (EA) at low frequency could effectively attenuate neuropathic pain. Our previous study showed that 2-Hz EA could relieve pain well in PDN. The study aimed to investigate whether 2-Hz EA relieves pain in PDN through suppressing PKC-mediated DRG P2X3 receptor upregulation. A 7-week feeding of high-fat and high-sugar diet plus a single injection of streptozotocin (STZ) in a dose of 35 mg/kg after a 5-week feeding of the diet successfully induced type 2 PDN in rats as revealed by the elevated body weight, fasting blood glucose, fasting insulin and insulin resistance, and the reduced paw withdrawal threshold (PWT), as well as the destructive ultrastructural change of sciatic nerve. DRG plasma membrane P2X3 receptor level and DRG PKC expression were elevated. Two-hertz EA failed to improve peripheral neuropathy; however, it reduced PWT, DRG plasma membrane P2X3 receptor level, and DRG PKC expression in PDN rats. Intraperitoneal administration of P2X3 receptor agonist αβ-meATP or PKC activator phorbol 12-myristate 13-acetate (PMA) blocked 2-Hz EA analgesia. Furthermore, PMA administration increased DRG plasma membrane P2X3 receptor level in PDN rats subject to 2-Hz EA treatment. These findings together indicated that the analgesic effect of EA in PDN is mediated by suppressing PKC-dependent membrane P2X3 upregulation in DRG. EA at low frequency is a valuable approach for PDN control. Springer Netherlands 2018-08-07 2018-12 /pmc/articles/PMC6298917/ /pubmed/30084084 http://dx.doi.org/10.1007/s11302-018-9617-4 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Zhou, Ya-feng
Ying, Xiao-ming
He, Xiao-fen
Shou, Sheng-Yun
Wei, Jun-Jun
Tai, Zhao-xia
Shao, Xiao-mei
Liang, Yi
Fang, Fang
Fang, Jian-qiao
Jiang, Yong-liang
Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy
title Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy
title_full Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy
title_fullStr Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy
title_full_unstemmed Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy
title_short Suppressing PKC-dependent membrane P2X3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy
title_sort suppressing pkc-dependent membrane p2x3 receptor upregulation in dorsal root ganglia mediated electroacupuncture analgesia in rat painful diabetic neuropathy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298917/
https://www.ncbi.nlm.nih.gov/pubmed/30084084
http://dx.doi.org/10.1007/s11302-018-9617-4
work_keys_str_mv AT zhouyafeng suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT yingxiaoming suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT hexiaofen suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT shoushengyun suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT weijunjun suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT taizhaoxia suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT shaoxiaomei suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT liangyi suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT fangfang suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT fangjianqiao suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy
AT jiangyongliang suppressingpkcdependentmembranep2x3receptorupregulationindorsalrootgangliamediatedelectroacupunctureanalgesiainratpainfuldiabeticneuropathy