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AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria
3′ untranslated regions (3′ UTRs) and particularly long 3′ UTRs have been shown to act as a new class of post-transcriptional regulatory element. We previously reported that hmsT mRNA stability is negatively regulated by the 3′ UTR of hmsT in Yersinia pestis. To investigate more general effects of 3...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299119/ https://www.ncbi.nlm.nih.gov/pubmed/30619162 http://dx.doi.org/10.3389/fmicb.2018.03080 |
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author | Zhao, Ju-Ping Zhu, Hui Guo, Xiao-Peng Sun, Yi-Cheng |
author_facet | Zhao, Ju-Ping Zhu, Hui Guo, Xiao-Peng Sun, Yi-Cheng |
author_sort | Zhao, Ju-Ping |
collection | PubMed |
description | 3′ untranslated regions (3′ UTRs) and particularly long 3′ UTRs have been shown to act as a new class of post-transcriptional regulatory element. We previously reported that hmsT mRNA stability is negatively regulated by the 3′ UTR of hmsT in Yersinia pestis. To investigate more general effects of 3′ UTRs in Y. pestis, we selected 15 genes potentially possessing long 3′ UTRs with different AU content and constructed their 3′ UTR deletion mutants. Deletion of AU-rich 3′ UTRs increased mRNA levels, whereas deletion of 3′ UTRs with normal AU content resulted in slight or no changes in the mRNA level. In addition, we found that PNPase was important for 3′ UTR-mediated mRNA decay when the transcriptional terminator was Rho-dependent. Finally, we showed that ribosomes promote mRNA stability when bound to a 3′ UTR. Our findings suggest that functional 3′ UTRs might be broadly distributed in bacteria and their novel regulatory mechanisms require further investigation. |
format | Online Article Text |
id | pubmed-6299119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62991192019-01-07 AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria Zhao, Ju-Ping Zhu, Hui Guo, Xiao-Peng Sun, Yi-Cheng Front Microbiol Microbiology 3′ untranslated regions (3′ UTRs) and particularly long 3′ UTRs have been shown to act as a new class of post-transcriptional regulatory element. We previously reported that hmsT mRNA stability is negatively regulated by the 3′ UTR of hmsT in Yersinia pestis. To investigate more general effects of 3′ UTRs in Y. pestis, we selected 15 genes potentially possessing long 3′ UTRs with different AU content and constructed their 3′ UTR deletion mutants. Deletion of AU-rich 3′ UTRs increased mRNA levels, whereas deletion of 3′ UTRs with normal AU content resulted in slight or no changes in the mRNA level. In addition, we found that PNPase was important for 3′ UTR-mediated mRNA decay when the transcriptional terminator was Rho-dependent. Finally, we showed that ribosomes promote mRNA stability when bound to a 3′ UTR. Our findings suggest that functional 3′ UTRs might be broadly distributed in bacteria and their novel regulatory mechanisms require further investigation. Frontiers Media S.A. 2018-12-12 /pmc/articles/PMC6299119/ /pubmed/30619162 http://dx.doi.org/10.3389/fmicb.2018.03080 Text en Copyright © 2018 Zhao, Zhu, Guo and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhao, Ju-Ping Zhu, Hui Guo, Xiao-Peng Sun, Yi-Cheng AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria |
title | AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria |
title_full | AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria |
title_fullStr | AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria |
title_full_unstemmed | AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria |
title_short | AU-Rich Long 3′ Untranslated Region Regulates Gene Expression in Bacteria |
title_sort | au-rich long 3′ untranslated region regulates gene expression in bacteria |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299119/ https://www.ncbi.nlm.nih.gov/pubmed/30619162 http://dx.doi.org/10.3389/fmicb.2018.03080 |
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