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Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi
Aphelids are little-known phagotrophic parasites of algae whose life cycle and morphology resemble those of the parasitic rozellids (Cryptomycota, Rozellomycota). In previous phylogenetic analyses of RNA polymerase and rRNA genes, aphelids, rozellids and Microsporidia (parasites of animals) formed a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299283/ https://www.ncbi.nlm.nih.gov/pubmed/30588510 http://dx.doi.org/10.1038/s42003-018-0235-z |
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author | Torruella, Guifré Grau-Bové, Xavier Moreira, David Karpov, Sergey A. Burns, John A. Sebé-Pedrós, Arnau Völcker, Eckhard López-García, Purificación |
author_facet | Torruella, Guifré Grau-Bové, Xavier Moreira, David Karpov, Sergey A. Burns, John A. Sebé-Pedrós, Arnau Völcker, Eckhard López-García, Purificación |
author_sort | Torruella, Guifré |
collection | PubMed |
description | Aphelids are little-known phagotrophic parasites of algae whose life cycle and morphology resemble those of the parasitic rozellids (Cryptomycota, Rozellomycota). In previous phylogenetic analyses of RNA polymerase and rRNA genes, aphelids, rozellids and Microsporidia (parasites of animals) formed a clade, named Opisthosporidia, which appeared as the sister group to Fungi. However, the statistical support for the Opisthosporidia was always moderate. Here, we generated full life-cycle transcriptome data for the aphelid species Paraphelidium tribonemae. In-depth multi-gene phylogenomic analyses using several protein datasets place this aphelid as the closest relative of fungi to the exclusion of rozellids and Microsporidia. In contrast with the comparatively reduced Rozella allomycis genome, we infer a rich, free-living-like aphelid proteome, with a metabolism similar to fungi, including cellulases likely involved in algal cell-wall penetration and enzymes involved in chitin biosynthesis. Our results suggest that fungi evolved from complex aphelid-like ancestors that lost phagotrophy and became osmotrophic. |
format | Online Article Text |
id | pubmed-6299283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62992832018-12-26 Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi Torruella, Guifré Grau-Bové, Xavier Moreira, David Karpov, Sergey A. Burns, John A. Sebé-Pedrós, Arnau Völcker, Eckhard López-García, Purificación Commun Biol Article Aphelids are little-known phagotrophic parasites of algae whose life cycle and morphology resemble those of the parasitic rozellids (Cryptomycota, Rozellomycota). In previous phylogenetic analyses of RNA polymerase and rRNA genes, aphelids, rozellids and Microsporidia (parasites of animals) formed a clade, named Opisthosporidia, which appeared as the sister group to Fungi. However, the statistical support for the Opisthosporidia was always moderate. Here, we generated full life-cycle transcriptome data for the aphelid species Paraphelidium tribonemae. In-depth multi-gene phylogenomic analyses using several protein datasets place this aphelid as the closest relative of fungi to the exclusion of rozellids and Microsporidia. In contrast with the comparatively reduced Rozella allomycis genome, we infer a rich, free-living-like aphelid proteome, with a metabolism similar to fungi, including cellulases likely involved in algal cell-wall penetration and enzymes involved in chitin biosynthesis. Our results suggest that fungi evolved from complex aphelid-like ancestors that lost phagotrophy and became osmotrophic. Nature Publishing Group UK 2018-12-19 /pmc/articles/PMC6299283/ /pubmed/30588510 http://dx.doi.org/10.1038/s42003-018-0235-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Torruella, Guifré Grau-Bové, Xavier Moreira, David Karpov, Sergey A. Burns, John A. Sebé-Pedrós, Arnau Völcker, Eckhard López-García, Purificación Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi |
title | Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi |
title_full | Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi |
title_fullStr | Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi |
title_full_unstemmed | Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi |
title_short | Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi |
title_sort | global transcriptome analysis of the aphelid paraphelidium tribonemae supports the phagotrophic origin of fungi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299283/ https://www.ncbi.nlm.nih.gov/pubmed/30588510 http://dx.doi.org/10.1038/s42003-018-0235-z |
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