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Carbapenemases on the move: it’s good to be on ICEs

BACKGROUND: The evolution and spread of antibiotic resistance is often mediated by mobile genetic elements. Integrative and conjugative elements (ICEs) are the most abundant conjugative elements among prokaryotes. However, the contribution of ICEs to horizontal gene transfer of antibiotic resistance...

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Autores principales: Botelho, João, Roberts, Adam P., León-Sampedro, Ricardo, Grosso, Filipa, Peixe, Luísa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299553/
https://www.ncbi.nlm.nih.gov/pubmed/30574213
http://dx.doi.org/10.1186/s13100-018-0141-4
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author Botelho, João
Roberts, Adam P.
León-Sampedro, Ricardo
Grosso, Filipa
Peixe, Luísa
author_facet Botelho, João
Roberts, Adam P.
León-Sampedro, Ricardo
Grosso, Filipa
Peixe, Luísa
author_sort Botelho, João
collection PubMed
description BACKGROUND: The evolution and spread of antibiotic resistance is often mediated by mobile genetic elements. Integrative and conjugative elements (ICEs) are the most abundant conjugative elements among prokaryotes. However, the contribution of ICEs to horizontal gene transfer of antibiotic resistance has been largely unexplored. RESULTS: Here we report that ICEs belonging to mating-pair formation (MPF) classes G and T are highly prevalent among the opportunistic pathogen Pseudomonas aeruginosa, contributing to the spread of carbapenemase-encoding genes (CEGs). Most CEGs of the MPF(G) class were encoded within class I integrons, which co-harbour genes conferring resistance to other antibiotics. The majority of the integrons were located within Tn3-like and composite transposons. Conserved attachment site could be predicted for the MPF(G) class ICEs. MPF(T) class ICEs carried the CEGs within composite transposons which were not associated with integrons. CONCLUSIONS: The data presented here provides a global snapshot of the different CEG-harbouring ICEs and sheds light on the underappreciated contribution of these elements to the evolution and dissemination of antibiotic resistance on P. aeruginosa. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13100-018-0141-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-62995532018-12-20 Carbapenemases on the move: it’s good to be on ICEs Botelho, João Roberts, Adam P. León-Sampedro, Ricardo Grosso, Filipa Peixe, Luísa Mob DNA Research BACKGROUND: The evolution and spread of antibiotic resistance is often mediated by mobile genetic elements. Integrative and conjugative elements (ICEs) are the most abundant conjugative elements among prokaryotes. However, the contribution of ICEs to horizontal gene transfer of antibiotic resistance has been largely unexplored. RESULTS: Here we report that ICEs belonging to mating-pair formation (MPF) classes G and T are highly prevalent among the opportunistic pathogen Pseudomonas aeruginosa, contributing to the spread of carbapenemase-encoding genes (CEGs). Most CEGs of the MPF(G) class were encoded within class I integrons, which co-harbour genes conferring resistance to other antibiotics. The majority of the integrons were located within Tn3-like and composite transposons. Conserved attachment site could be predicted for the MPF(G) class ICEs. MPF(T) class ICEs carried the CEGs within composite transposons which were not associated with integrons. CONCLUSIONS: The data presented here provides a global snapshot of the different CEG-harbouring ICEs and sheds light on the underappreciated contribution of these elements to the evolution and dissemination of antibiotic resistance on P. aeruginosa. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13100-018-0141-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-19 /pmc/articles/PMC6299553/ /pubmed/30574213 http://dx.doi.org/10.1186/s13100-018-0141-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Botelho, João
Roberts, Adam P.
León-Sampedro, Ricardo
Grosso, Filipa
Peixe, Luísa
Carbapenemases on the move: it’s good to be on ICEs
title Carbapenemases on the move: it’s good to be on ICEs
title_full Carbapenemases on the move: it’s good to be on ICEs
title_fullStr Carbapenemases on the move: it’s good to be on ICEs
title_full_unstemmed Carbapenemases on the move: it’s good to be on ICEs
title_short Carbapenemases on the move: it’s good to be on ICEs
title_sort carbapenemases on the move: it’s good to be on ices
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299553/
https://www.ncbi.nlm.nih.gov/pubmed/30574213
http://dx.doi.org/10.1186/s13100-018-0141-4
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