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Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery
Background: The accurate determination of liver segment anatomy is essential to perform liver resection without complications and to ensure long-term outcomes after this operation. There are several perioperative methods for segment identification and surgical navigation, such as intraoperative ultr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299692/ https://www.ncbi.nlm.nih.gov/pubmed/30613285 http://dx.doi.org/10.7150/thno.29223 |
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author | Qian, Baifeng Kyuno, Daisuke Schäfer, Michael Gross, Wolfgang Mehrabi, Arianeb Ryschich, Eduard |
author_facet | Qian, Baifeng Kyuno, Daisuke Schäfer, Michael Gross, Wolfgang Mehrabi, Arianeb Ryschich, Eduard |
author_sort | Qian, Baifeng |
collection | PubMed |
description | Background: The accurate determination of liver segment anatomy is essential to perform liver resection without complications and to ensure long-term outcomes after this operation. There are several perioperative methods for segment identification and surgical navigation, such as intraoperative ultrasound, indigo carmine and near-infrared imaging with indocyanine green. The present study experimentally analyzed the usefulness of monocyte sequestration for liver segment labeling and imaging. Methods: Human monocytes were isolated from peripheral blood and directly or indirectly labeled with calcein or IRDye 800CW. Potential toxicity, labeling stability, and adhesion to ICAM-1 were analyzed in vitro. Monocyte sequestration in the liver microvasculature and liver segment labeling and boundary demarcation were studied using isolated mouse and pig liver perfusion and via intraportal injection in mouse liver tumor models. Results: The highest monocyte labeling efficiency was achieved using direct labeling with IRDye 800CW. Labeling was stable and did not influence cell viability. The labeled monocytes were highly sequestrated in the liver microvasculature, both after ex vivo perfusion and after injection in vivo , resulting in excellent labeling of selected liver segments and strong segment boundary demarcation. In contrast to results to a normal liver, monocyte sequestration was very low in tumor-associated blood vessels. Conclusions: The present experimental study shows that sequestration of labeled monocytes after superselective application demarcates the selected liver segment. These results illustrate potential of this technique for surgical navigation during liver surgery. |
format | Online Article Text |
id | pubmed-6299692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-62996922019-01-04 Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery Qian, Baifeng Kyuno, Daisuke Schäfer, Michael Gross, Wolfgang Mehrabi, Arianeb Ryschich, Eduard Theranostics Research Paper Background: The accurate determination of liver segment anatomy is essential to perform liver resection without complications and to ensure long-term outcomes after this operation. There are several perioperative methods for segment identification and surgical navigation, such as intraoperative ultrasound, indigo carmine and near-infrared imaging with indocyanine green. The present study experimentally analyzed the usefulness of monocyte sequestration for liver segment labeling and imaging. Methods: Human monocytes were isolated from peripheral blood and directly or indirectly labeled with calcein or IRDye 800CW. Potential toxicity, labeling stability, and adhesion to ICAM-1 were analyzed in vitro. Monocyte sequestration in the liver microvasculature and liver segment labeling and boundary demarcation were studied using isolated mouse and pig liver perfusion and via intraportal injection in mouse liver tumor models. Results: The highest monocyte labeling efficiency was achieved using direct labeling with IRDye 800CW. Labeling was stable and did not influence cell viability. The labeled monocytes were highly sequestrated in the liver microvasculature, both after ex vivo perfusion and after injection in vivo , resulting in excellent labeling of selected liver segments and strong segment boundary demarcation. In contrast to results to a normal liver, monocyte sequestration was very low in tumor-associated blood vessels. Conclusions: The present experimental study shows that sequestration of labeled monocytes after superselective application demarcates the selected liver segment. These results illustrate potential of this technique for surgical navigation during liver surgery. Ivyspring International Publisher 2018-11-29 /pmc/articles/PMC6299692/ /pubmed/30613285 http://dx.doi.org/10.7150/thno.29223 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Qian, Baifeng Kyuno, Daisuke Schäfer, Michael Gross, Wolfgang Mehrabi, Arianeb Ryschich, Eduard Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery |
title | Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery |
title_full | Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery |
title_fullStr | Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery |
title_full_unstemmed | Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery |
title_short | Liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery |
title_sort | liver segment imaging using monocyte sequestration: a potential tool for fluorescence-guided liver surgery |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299692/ https://www.ncbi.nlm.nih.gov/pubmed/30613285 http://dx.doi.org/10.7150/thno.29223 |
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