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Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells
Cells exhibit distinct 3D morphologies in vivo, and recapitulation of physiological cell morphologies in vitro is pivotal not only to elucidate many fundamental biological questions, but also to develop new approaches for tissue regeneration and drug screening. However, conventional cell culture met...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299721/ https://www.ncbi.nlm.nih.gov/pubmed/30581705 http://dx.doi.org/10.1002/advs.201801037 |
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author | Ma, Chi Chang, Bei Jing, Yan Kim, Harry Liu, Xiaohua |
author_facet | Ma, Chi Chang, Bei Jing, Yan Kim, Harry Liu, Xiaohua |
author_sort | Ma, Chi |
collection | PubMed |
description | Cells exhibit distinct 3D morphologies in vivo, and recapitulation of physiological cell morphologies in vitro is pivotal not only to elucidate many fundamental biological questions, but also to develop new approaches for tissue regeneration and drug screening. However, conventional cell culture methods in either a 2D petri dish or a 3D scaffold often lead to the loss of the physiological morphologies for many cells, such as bone cells (osteocytes) and dentinal cells (odontoblasts). Herein, a unique approach in developing a 3D extracellular matrix (ECM)‐like micropatterned synthetic matrix as a physiologically relevant 3D platform is reported to recapitulate the morphologies of osteocytes and odontoblasts in vitro. The bio‐inspired micropatterned matrix precisely mimics the hierarchic 3D nanofibrous tubular/canaliculi architecture as well as the compositions of the ECM of mineralized tissues, and is capable of controlling one single cell in a microisland of the matrix. Using this bio‐inspired 3D platform, individual bone and dental stem cells are successfully manipulated to recapitulate the physiological morphologies of osteocytes and odontoblasts in vitro, respectively. This work provides an excellent platform for an in‐depth understanding of cell–matrix interactions in 3D environments, paving the way for designing next‐generation biomaterials for tissue regeneration. |
format | Online Article Text |
id | pubmed-6299721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62997212018-12-21 Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells Ma, Chi Chang, Bei Jing, Yan Kim, Harry Liu, Xiaohua Adv Sci (Weinh) Communications Cells exhibit distinct 3D morphologies in vivo, and recapitulation of physiological cell morphologies in vitro is pivotal not only to elucidate many fundamental biological questions, but also to develop new approaches for tissue regeneration and drug screening. However, conventional cell culture methods in either a 2D petri dish or a 3D scaffold often lead to the loss of the physiological morphologies for many cells, such as bone cells (osteocytes) and dentinal cells (odontoblasts). Herein, a unique approach in developing a 3D extracellular matrix (ECM)‐like micropatterned synthetic matrix as a physiologically relevant 3D platform is reported to recapitulate the morphologies of osteocytes and odontoblasts in vitro. The bio‐inspired micropatterned matrix precisely mimics the hierarchic 3D nanofibrous tubular/canaliculi architecture as well as the compositions of the ECM of mineralized tissues, and is capable of controlling one single cell in a microisland of the matrix. Using this bio‐inspired 3D platform, individual bone and dental stem cells are successfully manipulated to recapitulate the physiological morphologies of osteocytes and odontoblasts in vitro, respectively. This work provides an excellent platform for an in‐depth understanding of cell–matrix interactions in 3D environments, paving the way for designing next‐generation biomaterials for tissue regeneration. John Wiley and Sons Inc. 2018-10-12 /pmc/articles/PMC6299721/ /pubmed/30581705 http://dx.doi.org/10.1002/advs.201801037 Text en © 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Communications Ma, Chi Chang, Bei Jing, Yan Kim, Harry Liu, Xiaohua Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells |
title | Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells |
title_full | Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells |
title_fullStr | Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells |
title_full_unstemmed | Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells |
title_short | Bio‐Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells |
title_sort | bio‐inspired micropatterned platforms recapitulate 3d physiological morphologies of bone and dentinal cells |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299721/ https://www.ncbi.nlm.nih.gov/pubmed/30581705 http://dx.doi.org/10.1002/advs.201801037 |
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