Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock

The enormous demand for new rootstock genotypes in Prunus spp. makes us to use micropropagation as an unavoidable propagation method. Therefore, the study on micropropagation of a new semi-dwarf vegetative rootstock namely Tetra (Prunus empyrean 3) was carried out to develop an optimized protocol. C...

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Detalles Bibliográficos
Autores principales: Sadeghi, F., Yadollahi, A., Kermani, M. Jafarkhani, Eftekhari, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Scientific Research and Technology, Egypt 2015
Materias:
Plant Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299739/
https://www.ncbi.nlm.nih.gov/pubmed/30647562
http://dx.doi.org/10.1016/j.jgeb.2014.12.006
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author Sadeghi, F.
Yadollahi, A.
Kermani, M. Jafarkhani
Eftekhari, M.
author_facet Sadeghi, F.
Yadollahi, A.
Kermani, M. Jafarkhani
Eftekhari, M.
author_sort Sadeghi, F.
collection PubMed
description The enormous demand for new rootstock genotypes in Prunus spp. makes us to use micropropagation as an unavoidable propagation method. Therefore, the study on micropropagation of a new semi-dwarf vegetative rootstock namely Tetra (Prunus empyrean 3) was carried out to develop an optimized protocol. Culture establishment using nodal segments was enhanced using WPM (woody plant medium) medium lacking growth regulators. From various shoot multiplication treatments, the highest number of shoots per explant (30.4) was found on ME (Media created specifically) medium supplemented with 0.8 mg l(−1) BAP and 0.05 mg l(−1) IBA. 100% in vitro rooting was achieved on ½ strength MS medium with 0.5 mg l(−1) IBA, 1.6 mg l(−1) thiamine and 150 mg l(−1) iron sequestrene.
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spelling pubmed-62997392019-01-15 Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock Sadeghi, F. Yadollahi, A. Kermani, M. Jafarkhani Eftekhari, M. J Genet Eng Biotechnol Plant Biotechnology The enormous demand for new rootstock genotypes in Prunus spp. makes us to use micropropagation as an unavoidable propagation method. Therefore, the study on micropropagation of a new semi-dwarf vegetative rootstock namely Tetra (Prunus empyrean 3) was carried out to develop an optimized protocol. Culture establishment using nodal segments was enhanced using WPM (woody plant medium) medium lacking growth regulators. From various shoot multiplication treatments, the highest number of shoots per explant (30.4) was found on ME (Media created specifically) medium supplemented with 0.8 mg l(−1) BAP and 0.05 mg l(−1) IBA. 100% in vitro rooting was achieved on ½ strength MS medium with 0.5 mg l(−1) IBA, 1.6 mg l(−1) thiamine and 150 mg l(−1) iron sequestrene. Academy of Scientific Research and Technology, Egypt 2015-06 2015-01-31 /pmc/articles/PMC6299739/ /pubmed/30647562 http://dx.doi.org/10.1016/j.jgeb.2014.12.006 Text en © 2015 Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Plant Biotechnology
Sadeghi, F.
Yadollahi, A.
Kermani, M. Jafarkhani
Eftekhari, M.
Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock
title Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock
title_full Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock
title_fullStr Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock
title_full_unstemmed Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock
title_short Optimizing culture media for in vitro proliferation and rooting of Tetra (Prunus empyrean 3) rootstock
title_sort optimizing culture media for in vitro proliferation and rooting of tetra (prunus empyrean 3) rootstock
topic Plant Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299739/
https://www.ncbi.nlm.nih.gov/pubmed/30647562
http://dx.doi.org/10.1016/j.jgeb.2014.12.006
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