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Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates
Bemisia tabaci (Gennadius) (Hemiptera, Aleyrodidae) is considered to be one of the most damaging pests in agriculture, causing severe losses in crops worldwide, affecting the tropical and subtropical regions. Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was used to assess th...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academy of Scientific Research and Technology, Egypt
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299814/ https://www.ncbi.nlm.nih.gov/pubmed/30647571 http://dx.doi.org/10.1016/j.jgeb.2014.12.004 |
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author | Fahmy, Inas Farouk Abou-Ali, Rania Mahmoud |
author_facet | Fahmy, Inas Farouk Abou-Ali, Rania Mahmoud |
author_sort | Fahmy, Inas Farouk |
collection | PubMed |
description | Bemisia tabaci (Gennadius) (Hemiptera, Aleyrodidae) is considered to be one of the most damaging pests in agriculture, causing severe losses in crops worldwide, affecting the tropical and subtropical regions. Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was used to assess the genetic diversity between different isolates collected from different regions in Egypt compared with some other worldwide isolates of this insect pest. Out of 12 primers 8 primers from Operon technology have shown to differentiate between 13 collected B. tabaci samples from all over Egypt and some other samples collected from different countries with two other populations representing biotypes A and B collected from the US used for biotype demarcation. Using 13 insect samples, RAPD analysis has produced a total number of 72 markers; about 68 polymorphic markers were revealed. The total number of bands obtained for each primer ranged from 4 to 14 within an average of 9 bands per primer. Of the pair wise combination among fifteen populations Ismailia population showed the highest similarity index (0.947), while US biotype A scored the lowest similarity index (0.326). Two major clusters were formed from the UPGMA dendrogram, which was constructed based on Dice similarity coefficient. RAPD-PCR screening demarcated the whitefly population based on the host species and genetic biotypes. Two major clusters have been revealed as A and B with two other minor clusters A1, A2, and B1, B2. Most of the samples collected from Egypt were clustered together in a minor cluster named A1. A1 group is divided into two sub-groups. A1a comprises the populations from Beni-Sweif in Upper Egypt, Ismailia, Kalyobia, El-Fayoum, Tanta, Kafr El-Sheikh, Alexandria, and A1b comprises Spain and Sudan. Group A1a is clustered together based on their host which belongs to the Cucurbitaceae family while Alexandria was separated individually based on its host which is cauliflower. Through the similarity matrix it could be concluded that the populations of Beni-Sweif, Ismailia, Kalyobia, El-Fayoum, Tanta, Kafr El-Sheikh had 80–90% similarity, while the Banha isolate had 30–40% similarity. |
format | Online Article Text |
id | pubmed-6299814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Academy of Scientific Research and Technology, Egypt |
record_format | MEDLINE/PubMed |
spelling | pubmed-62998142019-01-15 Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates Fahmy, Inas Farouk Abou-Ali, Rania Mahmoud J Genet Eng Biotechnol Genetic Diversity Bemisia tabaci (Gennadius) (Hemiptera, Aleyrodidae) is considered to be one of the most damaging pests in agriculture, causing severe losses in crops worldwide, affecting the tropical and subtropical regions. Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was used to assess the genetic diversity between different isolates collected from different regions in Egypt compared with some other worldwide isolates of this insect pest. Out of 12 primers 8 primers from Operon technology have shown to differentiate between 13 collected B. tabaci samples from all over Egypt and some other samples collected from different countries with two other populations representing biotypes A and B collected from the US used for biotype demarcation. Using 13 insect samples, RAPD analysis has produced a total number of 72 markers; about 68 polymorphic markers were revealed. The total number of bands obtained for each primer ranged from 4 to 14 within an average of 9 bands per primer. Of the pair wise combination among fifteen populations Ismailia population showed the highest similarity index (0.947), while US biotype A scored the lowest similarity index (0.326). Two major clusters were formed from the UPGMA dendrogram, which was constructed based on Dice similarity coefficient. RAPD-PCR screening demarcated the whitefly population based on the host species and genetic biotypes. Two major clusters have been revealed as A and B with two other minor clusters A1, A2, and B1, B2. Most of the samples collected from Egypt were clustered together in a minor cluster named A1. A1 group is divided into two sub-groups. A1a comprises the populations from Beni-Sweif in Upper Egypt, Ismailia, Kalyobia, El-Fayoum, Tanta, Kafr El-Sheikh, Alexandria, and A1b comprises Spain and Sudan. Group A1a is clustered together based on their host which belongs to the Cucurbitaceae family while Alexandria was separated individually based on its host which is cauliflower. Through the similarity matrix it could be concluded that the populations of Beni-Sweif, Ismailia, Kalyobia, El-Fayoum, Tanta, Kafr El-Sheikh had 80–90% similarity, while the Banha isolate had 30–40% similarity. Academy of Scientific Research and Technology, Egypt 2015-06 2015-01-24 /pmc/articles/PMC6299814/ /pubmed/30647571 http://dx.doi.org/10.1016/j.jgeb.2014.12.004 Text en © 2015 Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Genetic Diversity Fahmy, Inas Farouk Abou-Ali, Rania Mahmoud Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates |
title | Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates |
title_full | Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates |
title_fullStr | Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates |
title_full_unstemmed | Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates |
title_short | Studying genetic diversity of whitefly B. tabaci Egyptian isolates in relation to some worldwide isolates |
title_sort | studying genetic diversity of whitefly b. tabaci egyptian isolates in relation to some worldwide isolates |
topic | Genetic Diversity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299814/ https://www.ncbi.nlm.nih.gov/pubmed/30647571 http://dx.doi.org/10.1016/j.jgeb.2014.12.004 |
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