Prevalence and phylogenetic characterization of Listeria monocytogenes isolated from processed meat marketed in Egypt

Because of its high case fatality rate, listeriosis locates among the most frequent causes of death due to food-borne illness. In this study, a total of 150 processed meat samples were collected from Giza Governorate, Egypt. Phenotypic and genotypic identification of Listeria monocytogenes was perfo...

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Detalles Bibliográficos
Autores principales: Mohamed, Yasmin, Reda, Waffa W., Abdel-Moein, Khaled, Abd El-Razik, Khaled A., Barakat, Ashraf M.A., El Fadaly, Hassan A., Hassanain, Nawal A., Hegazi, Ahmed G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Scientific Research and Technology, Egypt 2016
Materias:
II : Microbial Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299886/
https://www.ncbi.nlm.nih.gov/pubmed/30647605
http://dx.doi.org/10.1016/j.jgeb.2016.06.001
Descripción
Sumario:Because of its high case fatality rate, listeriosis locates among the most frequent causes of death due to food-borne illness. In this study, a total of 150 processed meat samples were collected from Giza Governorate, Egypt. Phenotypic and genotypic identification of Listeria monocytogenes was performed using PCR incorporating listeriolysin O virulence gene hlyA followed by DNA sequence analysis. L. monocytogenes was confirmed in 4% of each of beef burger, minced meat, and luncheon samples. Phylogenetic analysis showed that all the six Egyptian isolates have high homology with Colombian isolate (EF030606), except one Egyptian isolate which showed high homology with Indian isolate (EU840690). The public health significance of these pathogens as well as recommended sanitary measures were discussed.

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