Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India

Fusarium oxysporum which causes wilt is a serious pathogen. Fusarium isolates were isolated from Assam located in North East region of India. Morphological identification of Fusarium isolates was done using conidial and hyphal structures. Molecular identification of Fusarium isolates was done by amp...

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Autores principales: Singha, Irom Manoj, Kakoty, Yelena, Unni, Bala Gopalan, Das, Jayshree, Kalita, Mohan Chandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Scientific Research and Technology, Egypt 2016
Materias:
II : Microbial Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299887/
https://www.ncbi.nlm.nih.gov/pubmed/30647603
http://dx.doi.org/10.1016/j.jgeb.2016.07.001
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author Singha, Irom Manoj
Kakoty, Yelena
Unni, Bala Gopalan
Das, Jayshree
Kalita, Mohan Chandra
author_facet Singha, Irom Manoj
Kakoty, Yelena
Unni, Bala Gopalan
Das, Jayshree
Kalita, Mohan Chandra
author_sort Singha, Irom Manoj
collection PubMed
description Fusarium oxysporum which causes wilt is a serious pathogen. Fusarium isolates were isolated from Assam located in North East region of India. Morphological identification of Fusarium isolates was done using conidial and hyphal structures. Molecular identification of Fusarium isolates was done by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal DNA using primers ITS1 and ITS4. All the ITS sequences were compared for gaps and similarity. Further, characterization of random amplified polymorphic DNA (RAPD) was carried out using 40 primers. 15 primers that gave reproducible results were selected. RAPD was used to observe the relatedness among these isolates. Thus, it was concluded that molecular profiling using ITS is an indispensable method for identification studies.
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spelling pubmed-62998872019-01-15 Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India Singha, Irom Manoj Kakoty, Yelena Unni, Bala Gopalan Das, Jayshree Kalita, Mohan Chandra J Genet Eng Biotechnol II : Microbial Biotechnology Fusarium oxysporum which causes wilt is a serious pathogen. Fusarium isolates were isolated from Assam located in North East region of India. Morphological identification of Fusarium isolates was done using conidial and hyphal structures. Molecular identification of Fusarium isolates was done by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal DNA using primers ITS1 and ITS4. All the ITS sequences were compared for gaps and similarity. Further, characterization of random amplified polymorphic DNA (RAPD) was carried out using 40 primers. 15 primers that gave reproducible results were selected. RAPD was used to observe the relatedness among these isolates. Thus, it was concluded that molecular profiling using ITS is an indispensable method for identification studies. Academy of Scientific Research and Technology, Egypt 2016-06 2016-07-25 /pmc/articles/PMC6299887/ /pubmed/30647603 http://dx.doi.org/10.1016/j.jgeb.2016.07.001 Text en © 2016 Production and hosting by Elsevier B.V. on behalf of Academy of Scientific Research & Technology. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle II : Microbial Biotechnology
Singha, Irom Manoj
Kakoty, Yelena
Unni, Bala Gopalan
Das, Jayshree
Kalita, Mohan Chandra
Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India
title Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India
title_full Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India
title_fullStr Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India
title_full_unstemmed Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India
title_short Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India
title_sort identification and characterization of fusarium sp. using its and rapd causing fusarium wilt of tomato isolated from assam, north east india
topic II : Microbial Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299887/
https://www.ncbi.nlm.nih.gov/pubmed/30647603
http://dx.doi.org/10.1016/j.jgeb.2016.07.001
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