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Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia
Context: Phytochemical and pharmacological data on Ducrosia anethifolia (DC.) Boiss. (Apiaceae), an Iranian medicinal plant, are scarce; however, furocoumarins are characteristic compounds of D. anethifolia. Objective: Our experiments identify the secondary metabolites of D. anethifolia and assess t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300088/ https://www.ncbi.nlm.nih.gov/pubmed/31070540 http://dx.doi.org/10.1080/13880209.2018.1548625 |
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author | Mottaghipisheh, Javad Nové, Márta Spengler, Gabriella Kúsz, Norbert Hohmann, Judit Csupor, Dezső |
author_facet | Mottaghipisheh, Javad Nové, Márta Spengler, Gabriella Kúsz, Norbert Hohmann, Judit Csupor, Dezső |
author_sort | Mottaghipisheh, Javad |
collection | PubMed |
description | Context: Phytochemical and pharmacological data on Ducrosia anethifolia (DC.) Boiss. (Apiaceae), an Iranian medicinal plant, are scarce; however, furocoumarins are characteristic compounds of D. anethifolia. Objective: Our experiments identify the secondary metabolites of D. anethifolia and assess their antitumor and anti-multidrug resistance activities. Materials and methods: Pure compounds were isolated from the extract of aerial parts of the plant by chromatographic methods. Bioactivities were tested on multidrug resistant and sensitive mouse T-lymphoma cell lines. The inhibition of the cancer MDR efflux pump ABCB1 was evaluated by flow cytometry (at 2 and 20 µM). A checkerboard microplate method was applied to study the interactions of furocoumarins and doxorubicin. Toxicity was studied using normal murine NIH/3T3 fibroblasts. Results: Thirteen pure compounds were isolated, nine furocoumarins namely, pabulenol (1), (+)-oxypeucedanin hydrate (2), oxypeucedanin (3), oxypeucedanin methanolate (4), (−)-oxypeucedanin hydrate (5), imperatorin (6), isogospherol (7), heraclenin (8), heraclenol (9), along with vanillic aldehyde (10), harmine (11), 3-hydroxy-α-ionone (12) and 2-C-methyl-erythrytol (13). Oxypeucedanin showed the highest in vitro antiproliferative and cytotoxic activity against parent (IC(50) = 25.98 ± 1.27, 40.33 ± 0.63 µM) and multidrug resistant cells (IC(50) = 28.89 ± 0.73, 66.68 ± 0.00 µM), respectively, and exhibited slight toxicity on normal murine fibroblasts (IC(50) = 57.18 ± 3.91 µM). Discussion and conclusions: Compounds 2, 3, 5, 7, 10–13 were identified for the first time from the Ducrosia genus. Here, we report a comprehensive in vitro assessment of the antitumor activities of D. anethifolia furocoumarins. Oxypeucedanin is a promising compound for further investigations for its anticancer effects. |
format | Online Article Text |
id | pubmed-6300088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-63000882018-12-20 Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia Mottaghipisheh, Javad Nové, Márta Spengler, Gabriella Kúsz, Norbert Hohmann, Judit Csupor, Dezső Pharm Biol Research Article Context: Phytochemical and pharmacological data on Ducrosia anethifolia (DC.) Boiss. (Apiaceae), an Iranian medicinal plant, are scarce; however, furocoumarins are characteristic compounds of D. anethifolia. Objective: Our experiments identify the secondary metabolites of D. anethifolia and assess their antitumor and anti-multidrug resistance activities. Materials and methods: Pure compounds were isolated from the extract of aerial parts of the plant by chromatographic methods. Bioactivities were tested on multidrug resistant and sensitive mouse T-lymphoma cell lines. The inhibition of the cancer MDR efflux pump ABCB1 was evaluated by flow cytometry (at 2 and 20 µM). A checkerboard microplate method was applied to study the interactions of furocoumarins and doxorubicin. Toxicity was studied using normal murine NIH/3T3 fibroblasts. Results: Thirteen pure compounds were isolated, nine furocoumarins namely, pabulenol (1), (+)-oxypeucedanin hydrate (2), oxypeucedanin (3), oxypeucedanin methanolate (4), (−)-oxypeucedanin hydrate (5), imperatorin (6), isogospherol (7), heraclenin (8), heraclenol (9), along with vanillic aldehyde (10), harmine (11), 3-hydroxy-α-ionone (12) and 2-C-methyl-erythrytol (13). Oxypeucedanin showed the highest in vitro antiproliferative and cytotoxic activity against parent (IC(50) = 25.98 ± 1.27, 40.33 ± 0.63 µM) and multidrug resistant cells (IC(50) = 28.89 ± 0.73, 66.68 ± 0.00 µM), respectively, and exhibited slight toxicity on normal murine fibroblasts (IC(50) = 57.18 ± 3.91 µM). Discussion and conclusions: Compounds 2, 3, 5, 7, 10–13 were identified for the first time from the Ducrosia genus. Here, we report a comprehensive in vitro assessment of the antitumor activities of D. anethifolia furocoumarins. Oxypeucedanin is a promising compound for further investigations for its anticancer effects. Taylor & Francis 2018-12-17 /pmc/articles/PMC6300088/ /pubmed/31070540 http://dx.doi.org/10.1080/13880209.2018.1548625 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mottaghipisheh, Javad Nové, Márta Spengler, Gabriella Kúsz, Norbert Hohmann, Judit Csupor, Dezső Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia |
title | Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia |
title_full | Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia |
title_fullStr | Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia |
title_full_unstemmed | Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia |
title_short | Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia |
title_sort | antiproliferative and cytotoxic activities of furocoumarins of ducrosia anethifolia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300088/ https://www.ncbi.nlm.nih.gov/pubmed/31070540 http://dx.doi.org/10.1080/13880209.2018.1548625 |
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