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A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature
The conserved Rho-family GTPase Cdc42 is a master regulator of polarity establishment in many cell types. Cdc42 becomes activated and concentrated in a region of the cell cortex, and recruits a variety of effector proteins to that site. In turn, many effectors participate in regulation of cytoskelet...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300207/ https://www.ncbi.nlm.nih.gov/pubmed/30566437 http://dx.doi.org/10.1371/journal.pone.0200863 |
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author | Daniels, Christine N. Zyla, Trevin R. Lew, Daniel J. |
author_facet | Daniels, Christine N. Zyla, Trevin R. Lew, Daniel J. |
author_sort | Daniels, Christine N. |
collection | PubMed |
description | The conserved Rho-family GTPase Cdc42 is a master regulator of polarity establishment in many cell types. Cdc42 becomes activated and concentrated in a region of the cell cortex, and recruits a variety of effector proteins to that site. In turn, many effectors participate in regulation of cytoskeletal elements in order to remodel the cytoskeleton in a polarized manner. The budding yeast Saccharomyces cerevisiae has served as a tractable model system for studies of cell polarity. In yeast cells, Cdc42 polarization involves a positive feedback loop in which effectors called p21-activated kinases (PAKs) act to recruit a Cdc42-directed guanine nucleotide exchange factor (GEF), generating more GTP-Cdc42 in areas that already have GTP-Cdc42. The GTPase-interacting components (GICs) Gic1 and Gic2 are also Cdc42 effectors, and have been implicated in regulation of the actin and septin cytoskeleton. However, we report that cells lacking GICs are primarily defective in polarizing Cdc42 itself, suggesting that they act upstream as well as downstream of Cdc42 in yeast. Our findings suggest that feedback pathways involving GTPase effectors may be more prevalent than had been appreciated. |
format | Online Article Text |
id | pubmed-6300207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63002072018-12-28 A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature Daniels, Christine N. Zyla, Trevin R. Lew, Daniel J. PLoS One Research Article The conserved Rho-family GTPase Cdc42 is a master regulator of polarity establishment in many cell types. Cdc42 becomes activated and concentrated in a region of the cell cortex, and recruits a variety of effector proteins to that site. In turn, many effectors participate in regulation of cytoskeletal elements in order to remodel the cytoskeleton in a polarized manner. The budding yeast Saccharomyces cerevisiae has served as a tractable model system for studies of cell polarity. In yeast cells, Cdc42 polarization involves a positive feedback loop in which effectors called p21-activated kinases (PAKs) act to recruit a Cdc42-directed guanine nucleotide exchange factor (GEF), generating more GTP-Cdc42 in areas that already have GTP-Cdc42. The GTPase-interacting components (GICs) Gic1 and Gic2 are also Cdc42 effectors, and have been implicated in regulation of the actin and septin cytoskeleton. However, we report that cells lacking GICs are primarily defective in polarizing Cdc42 itself, suggesting that they act upstream as well as downstream of Cdc42 in yeast. Our findings suggest that feedback pathways involving GTPase effectors may be more prevalent than had been appreciated. Public Library of Science 2018-12-19 /pmc/articles/PMC6300207/ /pubmed/30566437 http://dx.doi.org/10.1371/journal.pone.0200863 Text en © 2018 Daniels et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Daniels, Christine N. Zyla, Trevin R. Lew, Daniel J. A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature |
title | A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature |
title_full | A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature |
title_fullStr | A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature |
title_full_unstemmed | A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature |
title_short | A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature |
title_sort | role for gic1 and gic2 in cdc42 polarization at elevated temperature |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300207/ https://www.ncbi.nlm.nih.gov/pubmed/30566437 http://dx.doi.org/10.1371/journal.pone.0200863 |
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