Cargando…

Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis

Background: There is a recognized need for an improved diagnostic test to assess post-chemotherapeutic treatment outcome in visceral leishmaniasis (VL) and to diagnose post kala-azar dermal leishmaniasis (PKDL). We previously demonstrated by ELISA and a prototype novel rapid diagnostic test (RDT), t...

Descripción completa

Detalles Bibliográficos
Autores principales: Marlais, Tegwen, Bhattacharyya, Tapan, Singh, Om Prakash, Mertens, Pascal, Gilleman, Quentin, Thunissen, Caroline, Hinckel, Bruno C. Bremer, Pearson, Callum, Gardner, Bathsheba L., Airs, Stephanie, de la Roche, Marianne, Hayes, Kiera, Hafezi, Hannah, Falconar, Andrew K., Eisa, Osama, Saad, Alfarazdeg, Khanal, Basudha, Bhattarai, Narayan Raj, Rijal, Suman, Boelaert, Marleen, El-Safi, Sayda, Sundar, Shyam, Miles, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300496/
https://www.ncbi.nlm.nih.gov/pubmed/30619774
http://dx.doi.org/10.3389/fcimb.2018.00427
_version_ 1783381693599580160
author Marlais, Tegwen
Bhattacharyya, Tapan
Singh, Om Prakash
Mertens, Pascal
Gilleman, Quentin
Thunissen, Caroline
Hinckel, Bruno C. Bremer
Pearson, Callum
Gardner, Bathsheba L.
Airs, Stephanie
de la Roche, Marianne
Hayes, Kiera
Hafezi, Hannah
Falconar, Andrew K.
Eisa, Osama
Saad, Alfarazdeg
Khanal, Basudha
Bhattarai, Narayan Raj
Rijal, Suman
Boelaert, Marleen
El-Safi, Sayda
Sundar, Shyam
Miles, Michael A.
author_facet Marlais, Tegwen
Bhattacharyya, Tapan
Singh, Om Prakash
Mertens, Pascal
Gilleman, Quentin
Thunissen, Caroline
Hinckel, Bruno C. Bremer
Pearson, Callum
Gardner, Bathsheba L.
Airs, Stephanie
de la Roche, Marianne
Hayes, Kiera
Hafezi, Hannah
Falconar, Andrew K.
Eisa, Osama
Saad, Alfarazdeg
Khanal, Basudha
Bhattarai, Narayan Raj
Rijal, Suman
Boelaert, Marleen
El-Safi, Sayda
Sundar, Shyam
Miles, Michael A.
author_sort Marlais, Tegwen
collection PubMed
description Background: There is a recognized need for an improved diagnostic test to assess post-chemotherapeutic treatment outcome in visceral leishmaniasis (VL) and to diagnose post kala-azar dermal leishmaniasis (PKDL). We previously demonstrated by ELISA and a prototype novel rapid diagnostic test (RDT), that high anti-Leishmania IgG1 is associated with post-treatment relapse versus cure in VL. Methodology: Here, we further evaluate this novel, low-cost RDT, named VL Sero K-SeT, and ELISA for monitoring IgG1 levels in VL patients after treatment. IgG1 levels against L. donovani lysate were determined. We applied these assays to Indian sera from cured VL at 6 months post treatment as well as to relapse and PKDL patients. Sudanese sera from pre- and post-treatment and relapse were also tested. Results: Of 104 paired Indian sera taken before and after treatment for VL, when deemed clinically cured, 81 (77.9%) were positive by VL Sero K-SeT before treatment; by 6 months, 68 of these 81 (84.0%) had a negative or reduced RDT test line intensity. ELISAs differed in positivity rate between pre- and post-treatment (p = 0.0162). Twenty eight of 33 (84.8%) Indian samples taken at diagnosis of relapse were RDT positive. A comparison of Indian VL Sero K-SeT data from patients deemed cured and relapsed confirmed that there was a significant difference (p < 0.0001) in positivity rate for the two groups using this RDT. Ten of 17 (58.8%) Sudanese sera went from positive to negative or decreased VL Sero K-SeT at the end of 11–30 days of treatment. Forty nine of 63 (77.8%) PKDL samples from India were positive by VL Sero K-SeT. Conclusion: We have further shown the relevance of IgG1 in determining clinical status in VL patients. A positive VL Sero K-SeT may also be helpful in supporting diagnosis of PKDL. With further refinement, such as the use of specific antigens, the VL Sero K-SeT and/or IgG1 ELISA may be adjuncts to current VL control programmes.
format Online
Article
Text
id pubmed-6300496
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-63004962019-01-07 Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis Marlais, Tegwen Bhattacharyya, Tapan Singh, Om Prakash Mertens, Pascal Gilleman, Quentin Thunissen, Caroline Hinckel, Bruno C. Bremer Pearson, Callum Gardner, Bathsheba L. Airs, Stephanie de la Roche, Marianne Hayes, Kiera Hafezi, Hannah Falconar, Andrew K. Eisa, Osama Saad, Alfarazdeg Khanal, Basudha Bhattarai, Narayan Raj Rijal, Suman Boelaert, Marleen El-Safi, Sayda Sundar, Shyam Miles, Michael A. Front Cell Infect Microbiol Cellular and Infection Microbiology Background: There is a recognized need for an improved diagnostic test to assess post-chemotherapeutic treatment outcome in visceral leishmaniasis (VL) and to diagnose post kala-azar dermal leishmaniasis (PKDL). We previously demonstrated by ELISA and a prototype novel rapid diagnostic test (RDT), that high anti-Leishmania IgG1 is associated with post-treatment relapse versus cure in VL. Methodology: Here, we further evaluate this novel, low-cost RDT, named VL Sero K-SeT, and ELISA for monitoring IgG1 levels in VL patients after treatment. IgG1 levels against L. donovani lysate were determined. We applied these assays to Indian sera from cured VL at 6 months post treatment as well as to relapse and PKDL patients. Sudanese sera from pre- and post-treatment and relapse were also tested. Results: Of 104 paired Indian sera taken before and after treatment for VL, when deemed clinically cured, 81 (77.9%) were positive by VL Sero K-SeT before treatment; by 6 months, 68 of these 81 (84.0%) had a negative or reduced RDT test line intensity. ELISAs differed in positivity rate between pre- and post-treatment (p = 0.0162). Twenty eight of 33 (84.8%) Indian samples taken at diagnosis of relapse were RDT positive. A comparison of Indian VL Sero K-SeT data from patients deemed cured and relapsed confirmed that there was a significant difference (p < 0.0001) in positivity rate for the two groups using this RDT. Ten of 17 (58.8%) Sudanese sera went from positive to negative or decreased VL Sero K-SeT at the end of 11–30 days of treatment. Forty nine of 63 (77.8%) PKDL samples from India were positive by VL Sero K-SeT. Conclusion: We have further shown the relevance of IgG1 in determining clinical status in VL patients. A positive VL Sero K-SeT may also be helpful in supporting diagnosis of PKDL. With further refinement, such as the use of specific antigens, the VL Sero K-SeT and/or IgG1 ELISA may be adjuncts to current VL control programmes. Frontiers Media S.A. 2018-12-13 /pmc/articles/PMC6300496/ /pubmed/30619774 http://dx.doi.org/10.3389/fcimb.2018.00427 Text en Copyright © 2018 Marlais, Bhattacharyya, Singh, Mertens, Gilleman, Thunissen, Hinckel, Pearson, Gardner, Airs, de la Roche, Hayes, Hafezi, Falconar, Eisa, Saad, Khanal, Bhattarai, Rijal, Boelaert, El-Safi, Sundar and Miles. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Marlais, Tegwen
Bhattacharyya, Tapan
Singh, Om Prakash
Mertens, Pascal
Gilleman, Quentin
Thunissen, Caroline
Hinckel, Bruno C. Bremer
Pearson, Callum
Gardner, Bathsheba L.
Airs, Stephanie
de la Roche, Marianne
Hayes, Kiera
Hafezi, Hannah
Falconar, Andrew K.
Eisa, Osama
Saad, Alfarazdeg
Khanal, Basudha
Bhattarai, Narayan Raj
Rijal, Suman
Boelaert, Marleen
El-Safi, Sayda
Sundar, Shyam
Miles, Michael A.
Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis
title Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis
title_full Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis
title_fullStr Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis
title_full_unstemmed Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis
title_short Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis
title_sort visceral leishmaniasis igg1 rapid monitoring of cure vs. relapse, and potential for diagnosis of post kala-azar dermal leishmaniasis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300496/
https://www.ncbi.nlm.nih.gov/pubmed/30619774
http://dx.doi.org/10.3389/fcimb.2018.00427
work_keys_str_mv AT marlaistegwen visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT bhattacharyyatapan visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT singhomprakash visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT mertenspascal visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT gillemanquentin visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT thunissencaroline visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT hinckelbrunocbremer visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT pearsoncallum visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT gardnerbathshebal visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT airsstephanie visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT delarochemarianne visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT hayeskiera visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT hafezihannah visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT falconarandrewk visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT eisaosama visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT saadalfarazdeg visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT khanalbasudha visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT bhattarainarayanraj visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT rijalsuman visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT boelaertmarleen visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT elsafisayda visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT sundarshyam visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis
AT milesmichaela visceralleishmaniasisigg1rapidmonitoringofcurevsrelapseandpotentialfordiagnosisofpostkalaazardermalleishmaniasis