Cargando…

A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products

We developed a modified Quick, Easy, Cheap, Effective, Rugged, and Safe (CEN QuEChERS) extraction method coupled with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI(+)/MS-MS) to identify and quantify residues of three botanical alkaloids, namely, scopolamine, L-hyoscy...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Weijia, Yoo, Kyung-Hee, Choi, Jeong-Min, Park, Da-Hee, Kim, Seong-Kwan, Kang, Young-Sun, Abd El-Aty, A.M., Hacımüftüoğlu, Ahmet, Jeong, Ji Hoon, Bekhit, Alaa El-Din, Shim, Jae-Han, Shin, Ho-Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300569/
https://www.ncbi.nlm.nih.gov/pubmed/30581617
http://dx.doi.org/10.1016/j.jare.2018.09.004
_version_ 1783381701801541632
author Zheng, Weijia
Yoo, Kyung-Hee
Choi, Jeong-Min
Park, Da-Hee
Kim, Seong-Kwan
Kang, Young-Sun
Abd El-Aty, A.M.
Hacımüftüoğlu, Ahmet
Jeong, Ji Hoon
Bekhit, Alaa El-Din
Shim, Jae-Han
Shin, Ho-Chul
author_facet Zheng, Weijia
Yoo, Kyung-Hee
Choi, Jeong-Min
Park, Da-Hee
Kim, Seong-Kwan
Kang, Young-Sun
Abd El-Aty, A.M.
Hacımüftüoğlu, Ahmet
Jeong, Ji Hoon
Bekhit, Alaa El-Din
Shim, Jae-Han
Shin, Ho-Chul
author_sort Zheng, Weijia
collection PubMed
description We developed a modified Quick, Easy, Cheap, Effective, Rugged, and Safe (CEN QuEChERS) extraction method coupled with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI(+)/MS-MS) to identify and quantify residues of three botanical alkaloids, namely, scopolamine, L-hyoscyamine, and sparteine, in animal-derived foods, including porcine muscle, egg, and milk. A combination of ethylenediaminetetraacetic acid disodium buffer and acetonitrile acidified with 0.5% trifluoroacetic acid was used as an extraction solvent, whereas QuEChERS (CEN, 15662) kits and sorbents were applied for cleanup procedures. The proposed method was validated by determining the limits of quantification (LOQs), with values of 1–5 µg/kg achieved for the target analytes in various matrices. Linearity was estimated from matrix-matched calibration curves constructed using six concentration levels ranging from 1- to 6-fold increases in the LOQs of each analyte, and the correlation coefficients (R(2)) were ≥0.9869. Recoveries (at three concentration levels of 1-, 2-, and 3-fold increases in the LOQ) of 73–104% were achieved with relative standard deviations (RSDs) ≤7.7% (intra-day and inter-day precision). Ten types of each matrix procured from large markets were evaluated, and all tested samples showed negative results. The current protocol is simple and versatile and can be used for routine detection of plant alkaloids in animal food products.
format Online
Article
Text
id pubmed-6300569
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-63005692018-12-21 A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products Zheng, Weijia Yoo, Kyung-Hee Choi, Jeong-Min Park, Da-Hee Kim, Seong-Kwan Kang, Young-Sun Abd El-Aty, A.M. Hacımüftüoğlu, Ahmet Jeong, Ji Hoon Bekhit, Alaa El-Din Shim, Jae-Han Shin, Ho-Chul J Adv Res Original Article We developed a modified Quick, Easy, Cheap, Effective, Rugged, and Safe (CEN QuEChERS) extraction method coupled with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI(+)/MS-MS) to identify and quantify residues of three botanical alkaloids, namely, scopolamine, L-hyoscyamine, and sparteine, in animal-derived foods, including porcine muscle, egg, and milk. A combination of ethylenediaminetetraacetic acid disodium buffer and acetonitrile acidified with 0.5% trifluoroacetic acid was used as an extraction solvent, whereas QuEChERS (CEN, 15662) kits and sorbents were applied for cleanup procedures. The proposed method was validated by determining the limits of quantification (LOQs), with values of 1–5 µg/kg achieved for the target analytes in various matrices. Linearity was estimated from matrix-matched calibration curves constructed using six concentration levels ranging from 1- to 6-fold increases in the LOQs of each analyte, and the correlation coefficients (R(2)) were ≥0.9869. Recoveries (at three concentration levels of 1-, 2-, and 3-fold increases in the LOQ) of 73–104% were achieved with relative standard deviations (RSDs) ≤7.7% (intra-day and inter-day precision). Ten types of each matrix procured from large markets were evaluated, and all tested samples showed negative results. The current protocol is simple and versatile and can be used for routine detection of plant alkaloids in animal food products. Elsevier 2018-09-27 /pmc/articles/PMC6300569/ /pubmed/30581617 http://dx.doi.org/10.1016/j.jare.2018.09.004 Text en © 2018 Production and hosting by Elsevier B.V. on behalf of Cairo University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zheng, Weijia
Yoo, Kyung-Hee
Choi, Jeong-Min
Park, Da-Hee
Kim, Seong-Kwan
Kang, Young-Sun
Abd El-Aty, A.M.
Hacımüftüoğlu, Ahmet
Jeong, Ji Hoon
Bekhit, Alaa El-Din
Shim, Jae-Han
Shin, Ho-Chul
A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products
title A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products
title_full A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products
title_fullStr A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products
title_full_unstemmed A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products
title_short A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products
title_sort modified quechers method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, l-hyoscyamine, and sparteine residues in animal-derived food products
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300569/
https://www.ncbi.nlm.nih.gov/pubmed/30581617
http://dx.doi.org/10.1016/j.jare.2018.09.004
work_keys_str_mv AT zhengweijia amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT yookyunghee amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT choijeongmin amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT parkdahee amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT kimseongkwan amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT kangyoungsun amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT abdelatyam amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT hacımuftuogluahmet amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT jeongjihoon amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT bekhitalaaeldin amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT shimjaehan amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT shinhochul amodifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT zhengweijia modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT yookyunghee modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT choijeongmin modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT parkdahee modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT kimseongkwan modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT kangyoungsun modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT abdelatyam modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT hacımuftuogluahmet modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT jeongjihoon modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT bekhitalaaeldin modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT shimjaehan modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts
AT shinhochul modifiedquechersmethodcoupledwithliquidchromatographytandemmassspectrometryforthesimultaneousdetectionandquantificationofscopolaminelhyoscyamineandsparteineresiduesinanimalderivedfoodproducts