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The Mono-Prep system increases the detection rate of sputum smear microscopy for diagnosing tuberculosis

OBJECTIVE: Direct sputum smear microscopy (DSSM) has a low detection rate. This study investigated whether an alternative method called Mono-Prep smear microscopy (MPSM) can enhance the diagnosis of tuberculosis in tuberculosis laboratories that perform direct smear microscopy in China. METHODS: A t...

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Detalles Bibliográficos
Autores principales: Wu, Jiannan, Kong, Chengcheng, Huo, Fengmin, Liang, Qian, Ma, Yifeng, Shang, Yuanyuan, Zhao, Liping, Du, Jian, Sun, Zhaogang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300963/
https://www.ncbi.nlm.nih.gov/pubmed/30178688
http://dx.doi.org/10.1177/0300060518792354
Descripción
Sumario:OBJECTIVE: Direct sputum smear microscopy (DSSM) has a low detection rate. This study investigated whether an alternative method called Mono-Prep smear microscopy (MPSM) can enhance the diagnosis of tuberculosis in tuberculosis laboratories that perform direct smear microscopy in China. METHODS: A total of 117 sputum samples were collected from outpatients who attended Beijing Chest Hospital. DSSM, MPSM, solid culture, and Xpert MTB/RIF were performed on the samples. RESULTS: The positive rates of DSSM, MPSM, solid culture, and Xpert MTB/RIF were 27.4% (32/117), 40.2% (47/117), 35.9% (42/117), and 52.1% (61/117), respectively. MPSM could detect 15 more cases of tuberculosis compared with DSSM (47 vs 32) among 117 sputum samples. This represented a significantly higher positive rate and sensitivity of MPSM compared with DSSM. However, MPSM appeared to have a lower specificity (81.3%) compared with DSSM (90.7%) with the solid culture used as a standard. CONCLUSION: Use of MPSM can increase the number of positive sputum samples, but it still needs improvement.