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Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes
We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Royal Society of Chemistry
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301274/ https://www.ncbi.nlm.nih.gov/pubmed/30403202 http://dx.doi.org/10.1039/c8cc06309e |
| _version_ | 1783381805420773376 |
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| author | Tokunou, Yoshihide Chinotaikul, Punthira Hattori, Shingo Clarke, Thomas A. Shi, Liang Hashimoto, Kazuhito Ishii, Kazuyuki Okamoto, Akihiro |
| author_facet | Tokunou, Yoshihide Chinotaikul, Punthira Hattori, Shingo Clarke, Thomas A. Shi, Liang Hashimoto, Kazuhito Ishii, Kazuyuki Okamoto, Akihiro |
| author_sort | Tokunou, Yoshihide |
| collection | PubMed |
| description | We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics. |
| format | Online Article Text |
| id | pubmed-6301274 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| publisher | Royal Society of Chemistry |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-63012742019-01-15 Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes Tokunou, Yoshihide Chinotaikul, Punthira Hattori, Shingo Clarke, Thomas A. Shi, Liang Hashimoto, Kazuhito Ishii, Kazuyuki Okamoto, Akihiro Chem Commun (Camb) Chemistry We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics. Royal Society of Chemistry 2018-12-25 2018-11-07 /pmc/articles/PMC6301274/ /pubmed/30403202 http://dx.doi.org/10.1039/c8cc06309e Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
| spellingShingle | Chemistry Tokunou, Yoshihide Chinotaikul, Punthira Hattori, Shingo Clarke, Thomas A. Shi, Liang Hashimoto, Kazuhito Ishii, Kazuyuki Okamoto, Akihiro Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes |
| title | Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes
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| title_full | Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes
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| title_fullStr | Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes
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| title_full_unstemmed | Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes
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| title_short | Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes
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| title_sort | whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes |
| topic | Chemistry |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301274/ https://www.ncbi.nlm.nih.gov/pubmed/30403202 http://dx.doi.org/10.1039/c8cc06309e |
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