Evaluation of the effects of different stocking densities on the sediment microbial community of juvenile hybrid grouper (♀Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus) in recirculating aquaculture systems

Aquatic microorganisms are an important part of aquatic ecosystems because they are involved in nutrient cycling and water quality, eventually influencing fish productivity. However, at present, reports on the effect of stocking density on microorganisms in sediment samples in recirculating aquaculture systems (RAS) are relatively rare. In this study, the changes in the microbial community in an RAS were investigated under different stocking densities of juvenile hybrid grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus). Total DNA was extracted from the sediment samples, the 16S rDNA gene was amplified, and the bacterial community was analysed by Illumina high-throughput sequencing. We identified 741 OTUs from a total of 409,031 reads. Based on the analysis of bacterial composition, richness, diversity, bacterial 16S and rDNA gene abundance; sediment sample comparisons; and the existence of specific bacterial taxa within four densities, we concluded that the dominant phyla in all samples were similar and included: Proteobacteria, Bacteroidetes, Nitrospirae, Planctomycetes, Verrucomicrobia, and Chloroflexi. However, their relative distributions differed at different fish densities. Linear discriminant analysis further indicated that the stocking treatment influenced the sediment bacterial community. This study indicates that under RAS aquaculture, mode density is a factor regulating the microbial community, which provides insights into microbe management in RAS culture.