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Cloning and expression of L-asparaginase from Bacillus tequilensis PV9W and therapeutic efficacy of Solid Lipid Particle formulations against cancer

L-asparaginase, a therapeutic involved in cancer therapy, from Bacillus tequilensis PV9W (ansA gene) was cloned and over expressed in Escherichia coli BL21 (DE3), achieved the aim of maximizing the yield of the recombinant enzyme (6.02 ± 1.77 IU/mL) within 12 h. The native L-asparaginase of B. tequi...

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Detalles Bibliográficos
Autores principales:	Shakambari, Ganeshan, Sameer Kumar, Rai, Ashokkumar, Balasubramaniem, Ganesh, Venkatachalam, Vasantha, Vairathevar Sivasamy, Varalakshmi, Perumal
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6301963/ https://www.ncbi.nlm.nih.gov/pubmed/30573733 http://dx.doi.org/10.1038/s41598-018-36161-1

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