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High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy
The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical ro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302096/ https://www.ncbi.nlm.nih.gov/pubmed/30573732 http://dx.doi.org/10.1038/s41467-018-07823-5 |
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author | Kang, Byungjun Shin, Jisoo Park, Hyun-Ji Rhyou, Chanryeol Kang, Donyoung Lee, Shin-Jeong Yoon, Young-sup Cho, Seung-Woo Lee, Hyungsuk |
author_facet | Kang, Byungjun Shin, Jisoo Park, Hyun-Ji Rhyou, Chanryeol Kang, Donyoung Lee, Shin-Jeong Yoon, Young-sup Cho, Seung-Woo Lee, Hyungsuk |
author_sort | Kang, Byungjun |
collection | PubMed |
description | The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures. |
format | Online Article Text |
id | pubmed-6302096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63020962018-12-23 High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy Kang, Byungjun Shin, Jisoo Park, Hyun-Ji Rhyou, Chanryeol Kang, Donyoung Lee, Shin-Jeong Yoon, Young-sup Cho, Seung-Woo Lee, Hyungsuk Nat Commun Article The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery. Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function. Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels. Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors. The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue. Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures. Nature Publishing Group UK 2018-12-20 /pmc/articles/PMC6302096/ /pubmed/30573732 http://dx.doi.org/10.1038/s41467-018-07823-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kang, Byungjun Shin, Jisoo Park, Hyun-Ji Rhyou, Chanryeol Kang, Donyoung Lee, Shin-Jeong Yoon, Young-sup Cho, Seung-Woo Lee, Hyungsuk High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy |
title | High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy |
title_full | High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy |
title_fullStr | High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy |
title_full_unstemmed | High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy |
title_short | High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy |
title_sort | high-resolution acoustophoretic 3d cell patterning to construct functional collateral cylindroids for ischemia therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302096/ https://www.ncbi.nlm.nih.gov/pubmed/30573732 http://dx.doi.org/10.1038/s41467-018-07823-5 |
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