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Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece

BACKGROUND: Intergenotypic recombinant hepatitis C virus (HCV) strains emerge rarely during coinfection of the same individual with two HCV genotypes. Few recombinant HCV strains have been identified to date and only one, CRF01 2k/1b, has become a worldwide concern. This study reevaluated the genoty...

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Autores principales: Kassela, Katerina, Karakasiliotis, Ioannis, Kokkiou, Eleni, Souvalidou, Fani, Mimidis, Panayotis, Veletza, Stavroula, Panopoulou, Maria, Koskinas, John, Mimidis, Konstantinos, Mavromara, Penelope
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hellenic Society of Gastroenterology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302191/
https://www.ncbi.nlm.nih.gov/pubmed/30598597
http://dx.doi.org/10.20524/aog.2018.0322
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author Kassela, Katerina
Karakasiliotis, Ioannis
Kokkiou, Eleni
Souvalidou, Fani
Mimidis, Panayotis
Veletza, Stavroula
Panopoulou, Maria
Koskinas, John
Mimidis, Konstantinos
Mavromara, Penelope
author_facet Kassela, Katerina
Karakasiliotis, Ioannis
Kokkiou, Eleni
Souvalidou, Fani
Mimidis, Panayotis
Veletza, Stavroula
Panopoulou, Maria
Koskinas, John
Mimidis, Konstantinos
Mavromara, Penelope
author_sort Kassela, Katerina
collection PubMed
description BACKGROUND: Intergenotypic recombinant hepatitis C virus (HCV) strains emerge rarely during coinfection of the same individual with two HCV genotypes. Few recombinant HCV strains have been identified to date and only one, CRF01 2k/1b, has become a worldwide concern. This study reevaluated the genotyping of three HCV genotype 2 strains from a group of patients with an unusually low rate of sustained virological response after pegylated interferon/ribavirin treatment. In addition, genetic determinants of host interferon resistance were evaluated. METHODS: The HCV type 2 strains from the patients’ serum were subjected to partial sequencing of the core-E1, NS2, NS5A and NS5B regions by reverse transcription polymerase chain reaction. Furthermore, the IFNL3 rs12979860 and the IFNL4 rs368234815 single nucleotide polymorphisms were defined in two of the three patients. RESULTS: All three strains were phylogenetically related to the Russia-derived CRF01 2k/1b while they encompassed the exact same 2k/1b junction site within NS2. CONCLUSION: This is the first report of HCV 2k/1b recombinants in Greece and the greater area of the Balkans.
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spelling pubmed-63021912019-01-01 Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece Kassela, Katerina Karakasiliotis, Ioannis Kokkiou, Eleni Souvalidou, Fani Mimidis, Panayotis Veletza, Stavroula Panopoulou, Maria Koskinas, John Mimidis, Konstantinos Mavromara, Penelope Ann Gastroenterol Original Article BACKGROUND: Intergenotypic recombinant hepatitis C virus (HCV) strains emerge rarely during coinfection of the same individual with two HCV genotypes. Few recombinant HCV strains have been identified to date and only one, CRF01 2k/1b, has become a worldwide concern. This study reevaluated the genotyping of three HCV genotype 2 strains from a group of patients with an unusually low rate of sustained virological response after pegylated interferon/ribavirin treatment. In addition, genetic determinants of host interferon resistance were evaluated. METHODS: The HCV type 2 strains from the patients’ serum were subjected to partial sequencing of the core-E1, NS2, NS5A and NS5B regions by reverse transcription polymerase chain reaction. Furthermore, the IFNL3 rs12979860 and the IFNL4 rs368234815 single nucleotide polymorphisms were defined in two of the three patients. RESULTS: All three strains were phylogenetically related to the Russia-derived CRF01 2k/1b while they encompassed the exact same 2k/1b junction site within NS2. CONCLUSION: This is the first report of HCV 2k/1b recombinants in Greece and the greater area of the Balkans. Hellenic Society of Gastroenterology 2019 2018-11-02 /pmc/articles/PMC6302191/ /pubmed/30598597 http://dx.doi.org/10.20524/aog.2018.0322 Text en Copyright: © Hellenic Society of Gastroenterology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kassela, Katerina
Karakasiliotis, Ioannis
Kokkiou, Eleni
Souvalidou, Fani
Mimidis, Panayotis
Veletza, Stavroula
Panopoulou, Maria
Koskinas, John
Mimidis, Konstantinos
Mavromara, Penelope
Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece
title Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece
title_full Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece
title_fullStr Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece
title_full_unstemmed Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece
title_short Intergenotypic 2k/1b hepatitis C virus recombinants in the East Macedonia and Thrace region of Greece
title_sort intergenotypic 2k/1b hepatitis c virus recombinants in the east macedonia and thrace region of greece
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302191/
https://www.ncbi.nlm.nih.gov/pubmed/30598597
http://dx.doi.org/10.20524/aog.2018.0322
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