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A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken

BACKGROUND: Highly diversified in morphology and structure, feathers have evolved into various forms. Frizzle feathers, which result from a developmental defect of the feather, are observed in several domestic chicken breeds. The frizzle phenotype is consistent with incomplete dominance of a major g...

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Autores principales: Dong, Jing, He, Chuan, Wang, Zhibing, Li, Yanqing, Li, Shanshan, Tao, Lin, Chen, Jiebo, Li, Donghua, Yang, Fenxia, Li, Naibin, Zhang, Quan, Zhang, Li, Wang, Guangqin, Akinyemi, Fisayo, Meng, He, Du, Bingwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302451/
https://www.ncbi.nlm.nih.gov/pubmed/30572816
http://dx.doi.org/10.1186/s12711-018-0441-7
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author Dong, Jing
He, Chuan
Wang, Zhibing
Li, Yanqing
Li, Shanshan
Tao, Lin
Chen, Jiebo
Li, Donghua
Yang, Fenxia
Li, Naibin
Zhang, Quan
Zhang, Li
Wang, Guangqin
Akinyemi, Fisayo
Meng, He
Du, Bingwang
author_facet Dong, Jing
He, Chuan
Wang, Zhibing
Li, Yanqing
Li, Shanshan
Tao, Lin
Chen, Jiebo
Li, Donghua
Yang, Fenxia
Li, Naibin
Zhang, Quan
Zhang, Li
Wang, Guangqin
Akinyemi, Fisayo
Meng, He
Du, Bingwang
author_sort Dong, Jing
collection PubMed
description BACKGROUND: Highly diversified in morphology and structure, feathers have evolved into various forms. Frizzle feathers, which result from a developmental defect of the feather, are observed in several domestic chicken breeds. The frizzle phenotype is consistent with incomplete dominance of a major gene, but the molecular mechanisms that underlie this phenotype remain obscure. Kirin, a Chinese indigenous chicken breed that originated in the Guangdong province, is famous for its frizzle feathers. The KRT75 gene is considered as the dominant gene responsible for the frizzle trait in several chicken breeds, but this is not the case in the Kirin breed. Thus, the objective of our study was to investigate the genomic region and mutation responsible for this phenotype in this particular breed. RESULTS: A resource population was produced by crossing Kirin and Huaixiang chickens to produce F(1) and F(2) generations. DNA samples from 75 frizzle feather and normal feather individuals were sequenced with double-digest genotyping by sequencing (dd-GBS). After the detection of 525,561 high-quality variants, a genome-wide association analysis was carried out and the gene responsible for the frizzle phenotype was localized within the type II α-keratin cluster on chromosome 33. Sanger sequencing was used to screen for mutations in the exons of five genes of this type II α-keratin cluster. A 15-bp deletion in exon 3 of KRT75L4 that showed complete segregation with the frizzle phenotype was detected within the F(2) population. Transcriptome sequencing demonstrated that KRT75L4 was expressed but that the transcript was shorter in Kirin than in Huaixiang chickens. In addition, by using Sanger sequencing, we were able to confirm that the deletion was in complete linkage with frizzle feathers. CONCLUSIONS: A deletion in the KRT75L4 gene is responsible for the frizzle feather phenotype in the Kirin chicken. The identification of this mutation, which causes a developmental defect of avian integument appendages, will improve our understanding of the mechanisms that are involved in feather formation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12711-018-0441-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-63024512018-12-31 A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken Dong, Jing He, Chuan Wang, Zhibing Li, Yanqing Li, Shanshan Tao, Lin Chen, Jiebo Li, Donghua Yang, Fenxia Li, Naibin Zhang, Quan Zhang, Li Wang, Guangqin Akinyemi, Fisayo Meng, He Du, Bingwang Genet Sel Evol Short Communication BACKGROUND: Highly diversified in morphology and structure, feathers have evolved into various forms. Frizzle feathers, which result from a developmental defect of the feather, are observed in several domestic chicken breeds. The frizzle phenotype is consistent with incomplete dominance of a major gene, but the molecular mechanisms that underlie this phenotype remain obscure. Kirin, a Chinese indigenous chicken breed that originated in the Guangdong province, is famous for its frizzle feathers. The KRT75 gene is considered as the dominant gene responsible for the frizzle trait in several chicken breeds, but this is not the case in the Kirin breed. Thus, the objective of our study was to investigate the genomic region and mutation responsible for this phenotype in this particular breed. RESULTS: A resource population was produced by crossing Kirin and Huaixiang chickens to produce F(1) and F(2) generations. DNA samples from 75 frizzle feather and normal feather individuals were sequenced with double-digest genotyping by sequencing (dd-GBS). After the detection of 525,561 high-quality variants, a genome-wide association analysis was carried out and the gene responsible for the frizzle phenotype was localized within the type II α-keratin cluster on chromosome 33. Sanger sequencing was used to screen for mutations in the exons of five genes of this type II α-keratin cluster. A 15-bp deletion in exon 3 of KRT75L4 that showed complete segregation with the frizzle phenotype was detected within the F(2) population. Transcriptome sequencing demonstrated that KRT75L4 was expressed but that the transcript was shorter in Kirin than in Huaixiang chickens. In addition, by using Sanger sequencing, we were able to confirm that the deletion was in complete linkage with frizzle feathers. CONCLUSIONS: A deletion in the KRT75L4 gene is responsible for the frizzle feather phenotype in the Kirin chicken. The identification of this mutation, which causes a developmental defect of avian integument appendages, will improve our understanding of the mechanisms that are involved in feather formation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12711-018-0441-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-20 /pmc/articles/PMC6302451/ /pubmed/30572816 http://dx.doi.org/10.1186/s12711-018-0441-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Communication
Dong, Jing
He, Chuan
Wang, Zhibing
Li, Yanqing
Li, Shanshan
Tao, Lin
Chen, Jiebo
Li, Donghua
Yang, Fenxia
Li, Naibin
Zhang, Quan
Zhang, Li
Wang, Guangqin
Akinyemi, Fisayo
Meng, He
Du, Bingwang
A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken
title A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken
title_full A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken
title_fullStr A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken
title_full_unstemmed A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken
title_short A novel deletion in KRT75L4 mediates the frizzle trait in a Chinese indigenous chicken
title_sort novel deletion in krt75l4 mediates the frizzle trait in a chinese indigenous chicken
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302451/
https://www.ncbi.nlm.nih.gov/pubmed/30572816
http://dx.doi.org/10.1186/s12711-018-0441-7
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