Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins

We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more...

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Autores principales: Rockstroh, Alexandra, Barzon, Luisa, Kumbukgolla, Widuranga, Su, Hoang Xuan, Lizarazo, Erley, Vincenti-Gonzalez, Maria Fernanda, Tami, Adriana, Ornelas, Alice M.M., Aguiar, Renato Santana, Cadar, Daniel, Schmidt-Chanasit, Jonas, Ulbert, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2019
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302594/
https://www.ncbi.nlm.nih.gov/pubmed/30387417
http://dx.doi.org/10.3201/eid2501.180605
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author Rockstroh, Alexandra
Barzon, Luisa
Kumbukgolla, Widuranga
Su, Hoang Xuan
Lizarazo, Erley
Vincenti-Gonzalez, Maria Fernanda
Tami, Adriana
Ornelas, Alice M.M.
Aguiar, Renato Santana
Cadar, Daniel
Schmidt-Chanasit, Jonas
Ulbert, Sebastian
author_facet Rockstroh, Alexandra
Barzon, Luisa
Kumbukgolla, Widuranga
Su, Hoang Xuan
Lizarazo, Erley
Vincenti-Gonzalez, Maria Fernanda
Tami, Adriana
Ornelas, Alice M.M.
Aguiar, Renato Santana
Cadar, Daniel
Schmidt-Chanasit, Jonas
Ulbert, Sebastian
author_sort Rockstroh, Alexandra
collection PubMed
description We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more accurate reporting of epidemiologic findings.
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spelling pubmed-63025942019-01-01 Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins Rockstroh, Alexandra Barzon, Luisa Kumbukgolla, Widuranga Su, Hoang Xuan Lizarazo, Erley Vincenti-Gonzalez, Maria Fernanda Tami, Adriana Ornelas, Alice M.M. Aguiar, Renato Santana Cadar, Daniel Schmidt-Chanasit, Jonas Ulbert, Sebastian Emerg Infect Dis Dispatch We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% of samples from travelers and 86.5% of samples from residents of endemic regions; 97.1% corresponded to the serotype identified by PCR. This ELISA enables more accurate reporting of epidemiologic findings. Centers for Disease Control and Prevention 2019-01 /pmc/articles/PMC6302594/ /pubmed/30387417 http://dx.doi.org/10.3201/eid2501.180605 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Rockstroh, Alexandra
Barzon, Luisa
Kumbukgolla, Widuranga
Su, Hoang Xuan
Lizarazo, Erley
Vincenti-Gonzalez, Maria Fernanda
Tami, Adriana
Ornelas, Alice M.M.
Aguiar, Renato Santana
Cadar, Daniel
Schmidt-Chanasit, Jonas
Ulbert, Sebastian
Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins
title Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins
title_full Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins
title_fullStr Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins
title_full_unstemmed Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins
title_short Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins
title_sort dengue virus igm serotyping by elisa with recombinant mutant envelope proteins
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302594/
https://www.ncbi.nlm.nih.gov/pubmed/30387417
http://dx.doi.org/10.3201/eid2501.180605
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