Delivery of genome editing tools by bacterial extracellular vesicles

Here we propose to use bacterial cells as factories that generate EVs harboring both the RNP complex (or any other CRISPR‐mediated tool) and a specific ligand molecule. The ligand would allow specific binding of EVs to cells with the matching receptors, adding a level of specificity to the delivery,...

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Detalles Bibliográficos
Autores principales: Liu, Yue, Smid, Eddy J., Abee, Tjakko, Notebaart, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Crystal Ball
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302703/
https://www.ncbi.nlm.nih.gov/pubmed/30549228
http://dx.doi.org/10.1111/1751-7915.13356
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author Liu, Yue
Smid, Eddy J.
Abee, Tjakko
Notebaart, Richard A.
author_facet Liu, Yue
Smid, Eddy J.
Abee, Tjakko
Notebaart, Richard A.
author_sort Liu, Yue
collection PubMed
description Here we propose to use bacterial cells as factories that generate EVs harboring both the RNP complex (or any other CRISPR‐mediated tool) and a specific ligand molecule. The ligand would allow specific binding of EVs to cells with the matching receptors, adding a level of specificity to the delivery, hence stimulating precise genome editing.[Image: see text]
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spelling pubmed-63027032018-12-31 Delivery of genome editing tools by bacterial extracellular vesicles Liu, Yue Smid, Eddy J. Abee, Tjakko Notebaart, Richard A. Microb Biotechnol Crystal Ball Here we propose to use bacterial cells as factories that generate EVs harboring both the RNP complex (or any other CRISPR‐mediated tool) and a specific ligand molecule. The ligand would allow specific binding of EVs to cells with the matching receptors, adding a level of specificity to the delivery, hence stimulating precise genome editing.[Image: see text] John Wiley and Sons Inc. 2018-12-13 /pmc/articles/PMC6302703/ /pubmed/30549228 http://dx.doi.org/10.1111/1751-7915.13356 Text en © 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Crystal Ball
Liu, Yue
Smid, Eddy J.
Abee, Tjakko
Notebaart, Richard A.
Delivery of genome editing tools by bacterial extracellular vesicles
title Delivery of genome editing tools by bacterial extracellular vesicles
title_full Delivery of genome editing tools by bacterial extracellular vesicles
title_fullStr Delivery of genome editing tools by bacterial extracellular vesicles
title_full_unstemmed Delivery of genome editing tools by bacterial extracellular vesicles
title_short Delivery of genome editing tools by bacterial extracellular vesicles
title_sort delivery of genome editing tools by bacterial extracellular vesicles
topic Crystal Ball
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302703/
https://www.ncbi.nlm.nih.gov/pubmed/30549228
http://dx.doi.org/10.1111/1751-7915.13356
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AT abeetjakko deliveryofgenomeeditingtoolsbybacterialextracellularvesicles
AT notebaartricharda deliveryofgenomeeditingtoolsbybacterialextracellularvesicles

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