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The art of vector engineering: towards the construction of next‐generation genetic tools

When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. In the field of genetic engineering, the most important tool developed was the plasmid vector. This technology has been continuously...

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Autores principales: Nora, Luísa Czamanski, Westmann, Cauã Antunes, Martins‐Santana, Leonardo, Alves, Luana de Fátima, Monteiro, Lummy Maria Oliveira, Guazzaroni, María‐Eugenia, Silva‐Rocha, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302727/
https://www.ncbi.nlm.nih.gov/pubmed/30259693
http://dx.doi.org/10.1111/1751-7915.13318
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author Nora, Luísa Czamanski
Westmann, Cauã Antunes
Martins‐Santana, Leonardo
Alves, Luana de Fátima
Monteiro, Lummy Maria Oliveira
Guazzaroni, María‐Eugenia
Silva‐Rocha, Rafael
author_facet Nora, Luísa Czamanski
Westmann, Cauã Antunes
Martins‐Santana, Leonardo
Alves, Luana de Fátima
Monteiro, Lummy Maria Oliveira
Guazzaroni, María‐Eugenia
Silva‐Rocha, Rafael
author_sort Nora, Luísa Czamanski
collection PubMed
description When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. In the field of genetic engineering, the most important tool developed was the plasmid vector. This technology has been continuously expanding and undergoing adaptations. Here, we provide a detailed view following the evolution of vectors built throughout the years destined to study microorganisms and their peculiarities, including those whose genomes can only be revealed through metagenomics. We remark how synthetic biology became a turning point in designing these genetic tools to create meaningful innovations. We have placed special focus on the tools for engineering bacteria and fungi (both yeast and filamentous fungi) and those available to construct metagenomic libraries. Based on this overview, future goals would include the development of modular vectors bearing standardized parts and orthogonally designed circuits, a task not fully addressed thus far. Finally, we present some challenges that should be overcome to enable the next generation of vector design and ways to address it.
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spelling pubmed-63027272018-12-31 The art of vector engineering: towards the construction of next‐generation genetic tools Nora, Luísa Czamanski Westmann, Cauã Antunes Martins‐Santana, Leonardo Alves, Luana de Fátima Monteiro, Lummy Maria Oliveira Guazzaroni, María‐Eugenia Silva‐Rocha, Rafael Microb Biotechnol Minireviews When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. In the field of genetic engineering, the most important tool developed was the plasmid vector. This technology has been continuously expanding and undergoing adaptations. Here, we provide a detailed view following the evolution of vectors built throughout the years destined to study microorganisms and their peculiarities, including those whose genomes can only be revealed through metagenomics. We remark how synthetic biology became a turning point in designing these genetic tools to create meaningful innovations. We have placed special focus on the tools for engineering bacteria and fungi (both yeast and filamentous fungi) and those available to construct metagenomic libraries. Based on this overview, future goals would include the development of modular vectors bearing standardized parts and orthogonally designed circuits, a task not fully addressed thus far. Finally, we present some challenges that should be overcome to enable the next generation of vector design and ways to address it. John Wiley and Sons Inc. 2018-09-26 /pmc/articles/PMC6302727/ /pubmed/30259693 http://dx.doi.org/10.1111/1751-7915.13318 Text en © 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Minireviews
Nora, Luísa Czamanski
Westmann, Cauã Antunes
Martins‐Santana, Leonardo
Alves, Luana de Fátima
Monteiro, Lummy Maria Oliveira
Guazzaroni, María‐Eugenia
Silva‐Rocha, Rafael
The art of vector engineering: towards the construction of next‐generation genetic tools
title The art of vector engineering: towards the construction of next‐generation genetic tools
title_full The art of vector engineering: towards the construction of next‐generation genetic tools
title_fullStr The art of vector engineering: towards the construction of next‐generation genetic tools
title_full_unstemmed The art of vector engineering: towards the construction of next‐generation genetic tools
title_short The art of vector engineering: towards the construction of next‐generation genetic tools
title_sort art of vector engineering: towards the construction of next‐generation genetic tools
topic Minireviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302727/
https://www.ncbi.nlm.nih.gov/pubmed/30259693
http://dx.doi.org/10.1111/1751-7915.13318
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