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Application of the uridine auxotrophic host and synthetic nucleosides for a rapid selection of hydrolases from metagenomic libraries
A high‐throughput method (≥ 10(6) of clones can be analysed on a single agar plate) for the selection of ester‐hydrolysing enzymes was developed based on the uridine auxotrophy of Escherichia coli strain DH10B ΔpyrFEC and the acylated derivatives 2′,3′,5′‐O‐tri‐acetyluridine and 2′,3′,5′‐O‐tri‐hexan...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302743/ https://www.ncbi.nlm.nih.gov/pubmed/30302933 http://dx.doi.org/10.1111/1751-7915.13316 |