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No support for cryptic choice by ovarian fluid in an external fertilizer

Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr (Salvelinus alpinus). We physically separated the OF...

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Autores principales: Kleppe, Snøfrid April, Nordeide, Jarle T., Rudolfsen, Geir, Figenschou, Lars, Larsen, Berner, Reiss, Katrin, Folstad, Ivar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303707/
https://www.ncbi.nlm.nih.gov/pubmed/30598774
http://dx.doi.org/10.1002/ece3.4628
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author Kleppe, Snøfrid April
Nordeide, Jarle T.
Rudolfsen, Geir
Figenschou, Lars
Larsen, Berner
Reiss, Katrin
Folstad, Ivar
author_facet Kleppe, Snøfrid April
Nordeide, Jarle T.
Rudolfsen, Geir
Figenschou, Lars
Larsen, Berner
Reiss, Katrin
Folstad, Ivar
author_sort Kleppe, Snøfrid April
collection PubMed
description Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr (Salvelinus alpinus). We physically separated the OF from the eggs of reproductively active females and reintroduced either their own OF or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split‐brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual OFs. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of OF treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that OF plays an important role as medium for cryptic female choice in charr. Power analyses revealed that our sample size is large enough to detect medium‐sized changes in relative paternity (medium‐sized effect sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about OF's potential influence on paternity under sperm competition—even in charr.
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spelling pubmed-63037072018-12-31 No support for cryptic choice by ovarian fluid in an external fertilizer Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar Ecol Evol Original Research Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr (Salvelinus alpinus). We physically separated the OF from the eggs of reproductively active females and reintroduced either their own OF or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split‐brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual OFs. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of OF treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that OF plays an important role as medium for cryptic female choice in charr. Power analyses revealed that our sample size is large enough to detect medium‐sized changes in relative paternity (medium‐sized effect sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about OF's potential influence on paternity under sperm competition—even in charr. John Wiley and Sons Inc. 2018-11-08 /pmc/articles/PMC6303707/ /pubmed/30598774 http://dx.doi.org/10.1002/ece3.4628 Text en © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Kleppe, Snøfrid April
Nordeide, Jarle T.
Rudolfsen, Geir
Figenschou, Lars
Larsen, Berner
Reiss, Katrin
Folstad, Ivar
No support for cryptic choice by ovarian fluid in an external fertilizer
title No support for cryptic choice by ovarian fluid in an external fertilizer
title_full No support for cryptic choice by ovarian fluid in an external fertilizer
title_fullStr No support for cryptic choice by ovarian fluid in an external fertilizer
title_full_unstemmed No support for cryptic choice by ovarian fluid in an external fertilizer
title_short No support for cryptic choice by ovarian fluid in an external fertilizer
title_sort no support for cryptic choice by ovarian fluid in an external fertilizer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303707/
https://www.ncbi.nlm.nih.gov/pubmed/30598774
http://dx.doi.org/10.1002/ece3.4628
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