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RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia

OBJECTIVE: Using high-throughput RNA sequencing technology, this study aimed to sequence the transcriptome of kidney and liver tissues harvested from Peninsular Malaysia cynomolgus macaque (Macaca fascicularis). M. fascicularis are significant nonhuman primate models in the biomedical field, owing t...

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Autores principales: Ee Uli, Joey, Yong, Christina Seok-Yien, Yeap, Swee Keong, Alitheen, Noorjahan Banu, Rovie-Ryan, Jeffrine J., Mat Isa, Nurulfiza, Tan, Soon Guan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303865/
https://www.ncbi.nlm.nih.gov/pubmed/30577850
http://dx.doi.org/10.1186/s13104-018-4014-1
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author Ee Uli, Joey
Yong, Christina Seok-Yien
Yeap, Swee Keong
Alitheen, Noorjahan Banu
Rovie-Ryan, Jeffrine J.
Mat Isa, Nurulfiza
Tan, Soon Guan
author_facet Ee Uli, Joey
Yong, Christina Seok-Yien
Yeap, Swee Keong
Alitheen, Noorjahan Banu
Rovie-Ryan, Jeffrine J.
Mat Isa, Nurulfiza
Tan, Soon Guan
author_sort Ee Uli, Joey
collection PubMed
description OBJECTIVE: Using high-throughput RNA sequencing technology, this study aimed to sequence the transcriptome of kidney and liver tissues harvested from Peninsular Malaysia cynomolgus macaque (Macaca fascicularis). M. fascicularis are significant nonhuman primate models in the biomedical field, owing to the macaque’s biological similarities with humans. The additional transcriptomic dataset will supplement the previously described Peninsular Malaysia M. fascicularis transcriptomes obtained in a past endeavour. RESULTS: A total of 75,350,240 sequence reads were obtained via Hi-seq 2500 sequencing technology. A total of 5473 significant differentially expressed genes were called. Gene ontology functional categorisation showed that cellular process, catalytic activity, and cell part categories had the highest number of expressed genes, while the metabolic pathways category possessed the highest number of expressed genes in the KEGG pathway analysis. The additional sequence dataset will further enrich existing M. fascicularis transcriptome assemblies, and provide a dataset for further downstream studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-4014-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-63038652018-12-31 RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia Ee Uli, Joey Yong, Christina Seok-Yien Yeap, Swee Keong Alitheen, Noorjahan Banu Rovie-Ryan, Jeffrine J. Mat Isa, Nurulfiza Tan, Soon Guan BMC Res Notes Research Note OBJECTIVE: Using high-throughput RNA sequencing technology, this study aimed to sequence the transcriptome of kidney and liver tissues harvested from Peninsular Malaysia cynomolgus macaque (Macaca fascicularis). M. fascicularis are significant nonhuman primate models in the biomedical field, owing to the macaque’s biological similarities with humans. The additional transcriptomic dataset will supplement the previously described Peninsular Malaysia M. fascicularis transcriptomes obtained in a past endeavour. RESULTS: A total of 75,350,240 sequence reads were obtained via Hi-seq 2500 sequencing technology. A total of 5473 significant differentially expressed genes were called. Gene ontology functional categorisation showed that cellular process, catalytic activity, and cell part categories had the highest number of expressed genes, while the metabolic pathways category possessed the highest number of expressed genes in the KEGG pathway analysis. The additional sequence dataset will further enrich existing M. fascicularis transcriptome assemblies, and provide a dataset for further downstream studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-4014-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-22 /pmc/articles/PMC6303865/ /pubmed/30577850 http://dx.doi.org/10.1186/s13104-018-4014-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Ee Uli, Joey
Yong, Christina Seok-Yien
Yeap, Swee Keong
Alitheen, Noorjahan Banu
Rovie-Ryan, Jeffrine J.
Mat Isa, Nurulfiza
Tan, Soon Guan
RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia
title RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia
title_full RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia
title_fullStr RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia
title_full_unstemmed RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia
title_short RNA sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (Macaca fascicularis) originating from Peninsular Malaysia
title_sort rna sequencing of kidney and liver transcriptome obtained from wild cynomolgus macaque (macaca fascicularis) originating from peninsular malaysia
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303865/
https://www.ncbi.nlm.nih.gov/pubmed/30577850
http://dx.doi.org/10.1186/s13104-018-4014-1
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