Cargando…

Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153

BACKGROUND: Although the neuroprotective role of propofol has been identified recently, the regulatory mechanism associated with microRNAs (miRNAs/miRs) in neuronal cells remains to be poorly understood. We aimed to explore the regulatory mechanism of propofol in hypoxia-injured rat pheochromocytoma...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Mingwei, Sun, Haiyan, Pang, Jinlei, Guo, Xiangfei, Huo, Yansong, Wu, Xianhong, Liu, Yaguang, Ma, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303956/
https://www.ncbi.nlm.nih.gov/pubmed/30579328
http://dx.doi.org/10.1186/s12871-018-0660-z
_version_ 1783382265853640704
author He, Mingwei
Sun, Haiyan
Pang, Jinlei
Guo, Xiangfei
Huo, Yansong
Wu, Xianhong
Liu, Yaguang
Ma, Jun
author_facet He, Mingwei
Sun, Haiyan
Pang, Jinlei
Guo, Xiangfei
Huo, Yansong
Wu, Xianhong
Liu, Yaguang
Ma, Jun
author_sort He, Mingwei
collection PubMed
description BACKGROUND: Although the neuroprotective role of propofol has been identified recently, the regulatory mechanism associated with microRNAs (miRNAs/miRs) in neuronal cells remains to be poorly understood. We aimed to explore the regulatory mechanism of propofol in hypoxia-injured rat pheochromocytoma (PC-12) cells. METHODS: PC-12 cells were exposed to hypoxia, and cell viability and apoptosis were assessed by CCK-8 assay and flow cytometry assay/Western blot analysis, respectively. Effects of propofol on hypoxia-injured cells were measured, and the expression of miR-153 was determined by stem-loop RT-PCR. After that, whether propofol affected PC-12 cells under hypoxia via miR-153 was verified, and the downstream protein of miR-153 as well as the involved signaling cascade was finally explored. RESULTS: Hypoxia-induced decrease of cell viability and increase of apoptosis were attenuated by propofol. Then, we found hypoxia exposure up-regulated miR-153 expression, and the level of miR-153 was further elevated by propofol in hypoxia-injured PC-12 cells. Following experiments showed miR-153 inhibition reversed the effects of propofol on hypoxia-treated PC-12 cells. Afterwards, we found BTG3 expression was negatively regulated by miR-153 expression, and BTG3 overexpression inhibited the mTOR pathway and AMPK activation. Besides, hypoxia inhibited the mTOR pathway and AMPK, and these inhibitory effects could be attenuated by propofol. CONCLUSION: Propofol protected hypoxia-injured PC-12 cells through miR-153-mediataed down-regulation of BTG3. BTG3 could inhibit the mTOR pathway and AMPK activation.
format Online
Article
Text
id pubmed-6303956
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-63039562018-12-31 Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153 He, Mingwei Sun, Haiyan Pang, Jinlei Guo, Xiangfei Huo, Yansong Wu, Xianhong Liu, Yaguang Ma, Jun BMC Anesthesiol Research Article BACKGROUND: Although the neuroprotective role of propofol has been identified recently, the regulatory mechanism associated with microRNAs (miRNAs/miRs) in neuronal cells remains to be poorly understood. We aimed to explore the regulatory mechanism of propofol in hypoxia-injured rat pheochromocytoma (PC-12) cells. METHODS: PC-12 cells were exposed to hypoxia, and cell viability and apoptosis were assessed by CCK-8 assay and flow cytometry assay/Western blot analysis, respectively. Effects of propofol on hypoxia-injured cells were measured, and the expression of miR-153 was determined by stem-loop RT-PCR. After that, whether propofol affected PC-12 cells under hypoxia via miR-153 was verified, and the downstream protein of miR-153 as well as the involved signaling cascade was finally explored. RESULTS: Hypoxia-induced decrease of cell viability and increase of apoptosis were attenuated by propofol. Then, we found hypoxia exposure up-regulated miR-153 expression, and the level of miR-153 was further elevated by propofol in hypoxia-injured PC-12 cells. Following experiments showed miR-153 inhibition reversed the effects of propofol on hypoxia-treated PC-12 cells. Afterwards, we found BTG3 expression was negatively regulated by miR-153 expression, and BTG3 overexpression inhibited the mTOR pathway and AMPK activation. Besides, hypoxia inhibited the mTOR pathway and AMPK, and these inhibitory effects could be attenuated by propofol. CONCLUSION: Propofol protected hypoxia-injured PC-12 cells through miR-153-mediataed down-regulation of BTG3. BTG3 could inhibit the mTOR pathway and AMPK activation. BioMed Central 2018-12-22 /pmc/articles/PMC6303956/ /pubmed/30579328 http://dx.doi.org/10.1186/s12871-018-0660-z Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
He, Mingwei
Sun, Haiyan
Pang, Jinlei
Guo, Xiangfei
Huo, Yansong
Wu, Xianhong
Liu, Yaguang
Ma, Jun
Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153
title Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153
title_full Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153
title_fullStr Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153
title_full_unstemmed Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153
title_short Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153
title_sort propofol alleviates hypoxia-induced nerve injury in pc-12 cells by up-regulation of microrna-153
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303956/
https://www.ncbi.nlm.nih.gov/pubmed/30579328
http://dx.doi.org/10.1186/s12871-018-0660-z
work_keys_str_mv AT hemingwei propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153
AT sunhaiyan propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153
AT pangjinlei propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153
AT guoxiangfei propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153
AT huoyansong propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153
AT wuxianhong propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153
AT liuyaguang propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153
AT majun propofolalleviateshypoxiainducednerveinjuryinpc12cellsbyupregulationofmicrorna153