Tuning recombinant protein expression to match secretion capacity

BACKGROUND: The secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activity. However, the secretion machinery of E. coli has a limited capacity and ca...

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Detalles Bibliográficos
Autores principales: Horga, Luminita Gabriela, Halliwell, Samantha, Castiñeiras, Tania Selas, Wyre, Chris, Matos, Cristina F. R. O., Yovcheva, Daniela S., Kent, Ross, Morra, Rosa, Williams, Steven G., Smith, Daniel C., Dixon, Neil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303999/
https://www.ncbi.nlm.nih.gov/pubmed/30577801
http://dx.doi.org/10.1186/s12934-018-1047-z

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