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NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model
The rapid production and release of a large number of inflammatory cytokines can cause excessive local and systemic inflammation in severe acute pancreatitis (SAP) and multiple organ dysfunction syndrome (MODS), especially pancreatitis-associated acute lung injury (P-ALI), which is the main cause of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304199/ https://www.ncbi.nlm.nih.gov/pubmed/30622955 http://dx.doi.org/10.1155/2018/1294951 |
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author | Fu, Qiang Zhai, Zhensheng Wang, Yuzhu Xu, Lixia Jia, Pengchong Xia, Peng Liu, Chuanjiang Zhang, Xu Qin, Tao Zhang, Hongwei |
author_facet | Fu, Qiang Zhai, Zhensheng Wang, Yuzhu Xu, Lixia Jia, Pengchong Xia, Peng Liu, Chuanjiang Zhang, Xu Qin, Tao Zhang, Hongwei |
author_sort | Fu, Qiang |
collection | PubMed |
description | The rapid production and release of a large number of inflammatory cytokines can cause excessive local and systemic inflammation in severe acute pancreatitis (SAP) and multiple organ dysfunction syndrome (MODS), especially pancreatitis-associated acute lung injury (P-ALI), which is the main cause of early death in patients with SAP. The NLRP3 inflammasome plays an important role in the maturation of IL-1β and the inflammatory cascade. Here, we established a model of SAP using wild-type (NLRP3(+/+)) and NLRP3 knockout (NLRP3(−/−)) mice by intraperitoneal injections of caerulein (Cae) and lipopolysaccharide (LPS). Pathological injury to the pancreas and lungs, the inflammatory response, and neutrophil infiltration were significantly mitigated in NLRP3(−/−) mice. Furthermore, INF-39, an NLRP3 inflammasome inhibitor, could reduce the severity of SAP and P-ALI in a dose-dependent manner. Our results suggested that SAP and P-ALI were alleviated by NLRP3 deficiency in mice, and thus, reducing NLRP3 expression may mitigate SAP-associated inflammation and P-ALI. |
format | Online Article Text |
id | pubmed-6304199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-63041992019-01-08 NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model Fu, Qiang Zhai, Zhensheng Wang, Yuzhu Xu, Lixia Jia, Pengchong Xia, Peng Liu, Chuanjiang Zhang, Xu Qin, Tao Zhang, Hongwei Biomed Res Int Research Article The rapid production and release of a large number of inflammatory cytokines can cause excessive local and systemic inflammation in severe acute pancreatitis (SAP) and multiple organ dysfunction syndrome (MODS), especially pancreatitis-associated acute lung injury (P-ALI), which is the main cause of early death in patients with SAP. The NLRP3 inflammasome plays an important role in the maturation of IL-1β and the inflammatory cascade. Here, we established a model of SAP using wild-type (NLRP3(+/+)) and NLRP3 knockout (NLRP3(−/−)) mice by intraperitoneal injections of caerulein (Cae) and lipopolysaccharide (LPS). Pathological injury to the pancreas and lungs, the inflammatory response, and neutrophil infiltration were significantly mitigated in NLRP3(−/−) mice. Furthermore, INF-39, an NLRP3 inflammasome inhibitor, could reduce the severity of SAP and P-ALI in a dose-dependent manner. Our results suggested that SAP and P-ALI were alleviated by NLRP3 deficiency in mice, and thus, reducing NLRP3 expression may mitigate SAP-associated inflammation and P-ALI. Hindawi 2018-11-28 /pmc/articles/PMC6304199/ /pubmed/30622955 http://dx.doi.org/10.1155/2018/1294951 Text en Copyright © 2018 Qiang Fu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Fu, Qiang Zhai, Zhensheng Wang, Yuzhu Xu, Lixia Jia, Pengchong Xia, Peng Liu, Chuanjiang Zhang, Xu Qin, Tao Zhang, Hongwei NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model |
title | NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model |
title_full | NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model |
title_fullStr | NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model |
title_full_unstemmed | NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model |
title_short | NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model |
title_sort | nlrp3 deficiency alleviates severe acute pancreatitis and pancreatitis-associated lung injury in a mouse model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304199/ https://www.ncbi.nlm.nih.gov/pubmed/30622955 http://dx.doi.org/10.1155/2018/1294951 |
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