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Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016
BACKGROUND: Six amino acid positions (145, 155, 156, 158, 159, and 189, referred to as the antigenic motif; H3 numbering) in the globular head region of hemagglutinin (HA1 domain) play an important role in defining the antigenic phenotype of swine Clade IV (C‐IV) H3N2 IAV, containing an H3 from a la...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304321/ https://www.ncbi.nlm.nih.gov/pubmed/30216671 http://dx.doi.org/10.1111/irv.12610 |
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author | Bolton, Marcus J. Abente, Eugenio J. Venkatesh, Divya Stratton, Jered A. Zeller, Michael Anderson, Tavis K. Lewis, Nicola S. Vincent, Amy L. |
author_facet | Bolton, Marcus J. Abente, Eugenio J. Venkatesh, Divya Stratton, Jered A. Zeller, Michael Anderson, Tavis K. Lewis, Nicola S. Vincent, Amy L. |
author_sort | Bolton, Marcus J. |
collection | PubMed |
description | BACKGROUND: Six amino acid positions (145, 155, 156, 158, 159, and 189, referred to as the antigenic motif; H3 numbering) in the globular head region of hemagglutinin (HA1 domain) play an important role in defining the antigenic phenotype of swine Clade IV (C‐IV) H3N2 IAV, containing an H3 from a late 1990s human‐to‐swine introduction. We hypothesized that antigenicity of a swine C‐IV H3 virus could be inferred based upon the antigenic motif if it matched a previously characterized antigen with the same motif. An increasing number of C‐IV H3 genes encoding antigenic motifs that had not been previously characterized were observed in the U.S. pig population between 2012 and 2016. OBJECTIVES: A broad panel of contemporary H3 viruses with uncharacterized antigenic motifs was selected across multiple clades within C‐IV to assess the impact of HA1 genetic diversity on the antigenic phenotype. METHODS: Hemagglutination inhibition (HI) assays were performed with isolates selected based on antigenic motif, tested against a panel of swine antisera, and visualized by antigenic cartography. RESULTS: A previously uncharacterized motif with low but sustained circulation in the swine population demonstrated a distinct phenotype from those previously characterized. Antigenic variation increased for viruses with similar antigenic motifs, likely due to amino acid substitutions outside the motif. CONCLUSIONS: Although antigenic motifs were largely associated with antigenic distances, substantial diversity among co‐circulating viruses poses a significant challenge for effective vaccine development. Continued surveillance and antigenic characterization of circulating strains is critical for improving vaccine efforts to control C‐IV H3 IAV in U.S. swine. |
format | Online Article Text |
id | pubmed-6304321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63043212019-01-04 Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016 Bolton, Marcus J. Abente, Eugenio J. Venkatesh, Divya Stratton, Jered A. Zeller, Michael Anderson, Tavis K. Lewis, Nicola S. Vincent, Amy L. Influenza Other Respir Viruses Original Articles BACKGROUND: Six amino acid positions (145, 155, 156, 158, 159, and 189, referred to as the antigenic motif; H3 numbering) in the globular head region of hemagglutinin (HA1 domain) play an important role in defining the antigenic phenotype of swine Clade IV (C‐IV) H3N2 IAV, containing an H3 from a late 1990s human‐to‐swine introduction. We hypothesized that antigenicity of a swine C‐IV H3 virus could be inferred based upon the antigenic motif if it matched a previously characterized antigen with the same motif. An increasing number of C‐IV H3 genes encoding antigenic motifs that had not been previously characterized were observed in the U.S. pig population between 2012 and 2016. OBJECTIVES: A broad panel of contemporary H3 viruses with uncharacterized antigenic motifs was selected across multiple clades within C‐IV to assess the impact of HA1 genetic diversity on the antigenic phenotype. METHODS: Hemagglutination inhibition (HI) assays were performed with isolates selected based on antigenic motif, tested against a panel of swine antisera, and visualized by antigenic cartography. RESULTS: A previously uncharacterized motif with low but sustained circulation in the swine population demonstrated a distinct phenotype from those previously characterized. Antigenic variation increased for viruses with similar antigenic motifs, likely due to amino acid substitutions outside the motif. CONCLUSIONS: Although antigenic motifs were largely associated with antigenic distances, substantial diversity among co‐circulating viruses poses a significant challenge for effective vaccine development. Continued surveillance and antigenic characterization of circulating strains is critical for improving vaccine efforts to control C‐IV H3 IAV in U.S. swine. John Wiley and Sons Inc. 2018-10-07 2019-01 /pmc/articles/PMC6304321/ /pubmed/30216671 http://dx.doi.org/10.1111/irv.12610 Text en © 2018 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Bolton, Marcus J. Abente, Eugenio J. Venkatesh, Divya Stratton, Jered A. Zeller, Michael Anderson, Tavis K. Lewis, Nicola S. Vincent, Amy L. Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016 |
title | Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016 |
title_full | Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016 |
title_fullStr | Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016 |
title_full_unstemmed | Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016 |
title_short | Antigenic evolution of H3N2 influenza A viruses in swine in the United States from 2012 to 2016 |
title_sort | antigenic evolution of h3n2 influenza a viruses in swine in the united states from 2012 to 2016 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304321/ https://www.ncbi.nlm.nih.gov/pubmed/30216671 http://dx.doi.org/10.1111/irv.12610 |
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