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Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR

In this study, 30 samples of processed edible mealworms (Tenebrio molitor L.) and 30 samples of grasshoppers (Locusta migratoria migratorioides) were obtained from producers located in Europe (Belgium and the Netherlands) and Asia (Thailand) and subjected to PCR-DGGE analyses. The PCR-DGGE analyses...

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Autores principales: Milanović, Vesna, Osimani, Andrea, Roncolini, Andrea, Garofalo, Cristiana, Aquilanti, Lucia, Pasquini, Marina, Tavoletti, Stefano, Vignaroli, Carla, Canonico, Laura, Ciani, Maurizio, Clementi, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304425/
https://www.ncbi.nlm.nih.gov/pubmed/30619127
http://dx.doi.org/10.3389/fmicb.2018.03036
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author Milanović, Vesna
Osimani, Andrea
Roncolini, Andrea
Garofalo, Cristiana
Aquilanti, Lucia
Pasquini, Marina
Tavoletti, Stefano
Vignaroli, Carla
Canonico, Laura
Ciani, Maurizio
Clementi, Francesca
author_facet Milanović, Vesna
Osimani, Andrea
Roncolini, Andrea
Garofalo, Cristiana
Aquilanti, Lucia
Pasquini, Marina
Tavoletti, Stefano
Vignaroli, Carla
Canonico, Laura
Ciani, Maurizio
Clementi, Francesca
author_sort Milanović, Vesna
collection PubMed
description In this study, 30 samples of processed edible mealworms (Tenebrio molitor L.) and 30 samples of grasshoppers (Locusta migratoria migratorioides) were obtained from producers located in Europe (Belgium and the Netherlands) and Asia (Thailand) and subjected to PCR-DGGE analyses. The PCR-DGGE analyses showed that species in the genus Staphylococcus were predominant in the samples of mealworms from Belgium and grasshoppers from the Netherlands; species in the genus Bacillus were detected in the samples of mealworms and grasshoppers from Thailand. Moreover, Weissella cibaria/confusa/spp. was found in grasshoppers from Belgium. Since data concerning the role of novel foods such as edible insects in the dissemination of carbapenem resistance are currently lacking, the quantification of five carbapenemase encoding genes (bla(NDM−1), bla(VIM), bla(GES), bla(OXA−48), and bla(KPC)) by qPCR was also carried out in all the samples under study. The genes coding for GES and KPC were not detected in the analyzed samples. A very low frequency of bla(OXA−48) (3%) and bla(NDM−1) (10%) genes was detected among mealworms. In contrast, grasshoppers were characterized by a high incidence of the genes for OXA-48 and NDM-1, accounting for 57 and 27% of the overall grasshopper samples, respectively. The bla(VIM) gene was detected exclusively in two grasshopper samples from Thailand, showing only 7% positivity. The analysis of variance showed that all the effects (producers, species, and producers × species) were statistically significant for bla(NDM−1), whereas for bla(OXA−48) and bla(VIM), no significant effects were detected for the same source of variation. Further studies are necessary to assess the possible role of edible insects as reservoirs for the resistance to carbapenems and to understand the correlation with the insect microbiota. Furthermore, an intensified surveillance plan examining the occurrence of carbapenemase encoding genes in the food chain and in environmental compartments is needed for a proper risk assessment. In such a context, the appropriate use of antimicrobials represents the main preventive action that should always be applied.
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spelling pubmed-63044252019-01-07 Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR Milanović, Vesna Osimani, Andrea Roncolini, Andrea Garofalo, Cristiana Aquilanti, Lucia Pasquini, Marina Tavoletti, Stefano Vignaroli, Carla Canonico, Laura Ciani, Maurizio Clementi, Francesca Front Microbiol Microbiology In this study, 30 samples of processed edible mealworms (Tenebrio molitor L.) and 30 samples of grasshoppers (Locusta migratoria migratorioides) were obtained from producers located in Europe (Belgium and the Netherlands) and Asia (Thailand) and subjected to PCR-DGGE analyses. The PCR-DGGE analyses showed that species in the genus Staphylococcus were predominant in the samples of mealworms from Belgium and grasshoppers from the Netherlands; species in the genus Bacillus were detected in the samples of mealworms and grasshoppers from Thailand. Moreover, Weissella cibaria/confusa/spp. was found in grasshoppers from Belgium. Since data concerning the role of novel foods such as edible insects in the dissemination of carbapenem resistance are currently lacking, the quantification of five carbapenemase encoding genes (bla(NDM−1), bla(VIM), bla(GES), bla(OXA−48), and bla(KPC)) by qPCR was also carried out in all the samples under study. The genes coding for GES and KPC were not detected in the analyzed samples. A very low frequency of bla(OXA−48) (3%) and bla(NDM−1) (10%) genes was detected among mealworms. In contrast, grasshoppers were characterized by a high incidence of the genes for OXA-48 and NDM-1, accounting for 57 and 27% of the overall grasshopper samples, respectively. The bla(VIM) gene was detected exclusively in two grasshopper samples from Thailand, showing only 7% positivity. The analysis of variance showed that all the effects (producers, species, and producers × species) were statistically significant for bla(NDM−1), whereas for bla(OXA−48) and bla(VIM), no significant effects were detected for the same source of variation. Further studies are necessary to assess the possible role of edible insects as reservoirs for the resistance to carbapenems and to understand the correlation with the insect microbiota. Furthermore, an intensified surveillance plan examining the occurrence of carbapenemase encoding genes in the food chain and in environmental compartments is needed for a proper risk assessment. In such a context, the appropriate use of antimicrobials represents the main preventive action that should always be applied. Frontiers Media S.A. 2018-12-17 /pmc/articles/PMC6304425/ /pubmed/30619127 http://dx.doi.org/10.3389/fmicb.2018.03036 Text en Copyright © 2018 Milanović, Osimani, Roncolini, Garofalo, Aquilanti, Pasquini, Tavoletti, Vignaroli, Canonico, Ciani and Clementi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Milanović, Vesna
Osimani, Andrea
Roncolini, Andrea
Garofalo, Cristiana
Aquilanti, Lucia
Pasquini, Marina
Tavoletti, Stefano
Vignaroli, Carla
Canonico, Laura
Ciani, Maurizio
Clementi, Francesca
Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR
title Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR
title_full Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR
title_fullStr Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR
title_full_unstemmed Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR
title_short Investigation of the Dominant Microbiota in Ready-to-Eat Grasshoppers and Mealworms and Quantification of Carbapenem Resistance Genes by qPCR
title_sort investigation of the dominant microbiota in ready-to-eat grasshoppers and mealworms and quantification of carbapenem resistance genes by qpcr
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304425/
https://www.ncbi.nlm.nih.gov/pubmed/30619127
http://dx.doi.org/10.3389/fmicb.2018.03036
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