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In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae
The aim of this study was to evaluate the in vitro lethal effect of a methanolic extract (ME) from Caesalpinia coriaria fruits against Haemonchus contortus eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME w...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304537/ https://www.ncbi.nlm.nih.gov/pubmed/30627567 http://dx.doi.org/10.1155/2018/7375693 |
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author | De Jesús-Martínez, X. Olmedo-Juárez, A. Olivares-Pérez, J. Zamilpa, A. Mendoza de Gives, P. López-Arellano, M. E. Rojas-Hernández, S. Villa-Mancera, A. Camacho-Díaz, L. M. Cipriano-Salazar, M. |
author_facet | De Jesús-Martínez, X. Olmedo-Juárez, A. Olivares-Pérez, J. Zamilpa, A. Mendoza de Gives, P. López-Arellano, M. E. Rojas-Hernández, S. Villa-Mancera, A. Camacho-Díaz, L. M. Cipriano-Salazar, M. |
author_sort | De Jesús-Martínez, X. |
collection | PubMed |
description | The aim of this study was to evaluate the in vitro lethal effect of a methanolic extract (ME) from Caesalpinia coriaria fruits against Haemonchus contortus eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME was assessed using five concentrations as follows: 6.15, 3.12, 1.56, and 0.78 mg/mL to eggs and 150, 100, 75, and 50 mg/mL to larvae, respectively. Ivermectin (5 mg/mL) was used as positive control and 4% methanol and distilled water were used as negative controls. The data of ovicidal and larvicidal effect were analyzed with a completely randomized design through ANOVA analysis using the general linear model (GLM) and lethal concentrations (LC(50) and LC(90)) were estimated through a Probit analysis using the SAS program. A clear ME increased concentration dependence effect was observed in the EHI and mortality tests. The highest activity of the methanolic extract was observed at the highest concentration (P < 0.05) to obtain a similar effect to the positive control (ivermectin), with LC(50) = 78.38 and 0.00064 mg/mL and LC(90) =235.63 and 0.024 mg/mL, respectively, for larvae and eggs. The results indicate that the C. coriaria fruit ME possesses in vitro ovicidal and larvicidal properties (gallotannins: methyl gallate) against H. contortus that needs to be investigated more in vivo for the control of gastroenteric nematodes in ruminants. |
format | Online Article Text |
id | pubmed-6304537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-63045372019-01-09 In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae De Jesús-Martínez, X. Olmedo-Juárez, A. Olivares-Pérez, J. Zamilpa, A. Mendoza de Gives, P. López-Arellano, M. E. Rojas-Hernández, S. Villa-Mancera, A. Camacho-Díaz, L. M. Cipriano-Salazar, M. Biomed Res Int Research Article The aim of this study was to evaluate the in vitro lethal effect of a methanolic extract (ME) from Caesalpinia coriaria fruits against Haemonchus contortus eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME was assessed using five concentrations as follows: 6.15, 3.12, 1.56, and 0.78 mg/mL to eggs and 150, 100, 75, and 50 mg/mL to larvae, respectively. Ivermectin (5 mg/mL) was used as positive control and 4% methanol and distilled water were used as negative controls. The data of ovicidal and larvicidal effect were analyzed with a completely randomized design through ANOVA analysis using the general linear model (GLM) and lethal concentrations (LC(50) and LC(90)) were estimated through a Probit analysis using the SAS program. A clear ME increased concentration dependence effect was observed in the EHI and mortality tests. The highest activity of the methanolic extract was observed at the highest concentration (P < 0.05) to obtain a similar effect to the positive control (ivermectin), with LC(50) = 78.38 and 0.00064 mg/mL and LC(90) =235.63 and 0.024 mg/mL, respectively, for larvae and eggs. The results indicate that the C. coriaria fruit ME possesses in vitro ovicidal and larvicidal properties (gallotannins: methyl gallate) against H. contortus that needs to be investigated more in vivo for the control of gastroenteric nematodes in ruminants. Hindawi 2018-11-29 /pmc/articles/PMC6304537/ /pubmed/30627567 http://dx.doi.org/10.1155/2018/7375693 Text en Copyright © 2018 X. De Jesús-Martínez et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article De Jesús-Martínez, X. Olmedo-Juárez, A. Olivares-Pérez, J. Zamilpa, A. Mendoza de Gives, P. López-Arellano, M. E. Rojas-Hernández, S. Villa-Mancera, A. Camacho-Díaz, L. M. Cipriano-Salazar, M. In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae |
title |
In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae |
title_full |
In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae |
title_fullStr |
In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae |
title_full_unstemmed |
In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae |
title_short |
In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae |
title_sort | in vitro anthelmintic activity of methanolic extract from caesalpinia coriaria j. willd fruits against haemonchus contortus eggs and infective larvae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304537/ https://www.ncbi.nlm.nih.gov/pubmed/30627567 http://dx.doi.org/10.1155/2018/7375693 |
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