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Imaging extracellular vesicles: current and emerging methods
Extracellular vesicles (EVs) are lipid bilayer-enclosed nanoparticles released by cells. They range from 30 nm to several micrometers in diameter, and ferry biological cargos such as proteins, lipids, RNAs and DNAs for local and distant intercellular communications. EVs have since been found to play...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304785/ https://www.ncbi.nlm.nih.gov/pubmed/30580764 http://dx.doi.org/10.1186/s12929-018-0494-5 |
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author | Chuo, Steven Ting-Yu Chien, Jasper Che-Yung Lai, Charles Pin-Kuang |
author_facet | Chuo, Steven Ting-Yu Chien, Jasper Che-Yung Lai, Charles Pin-Kuang |
author_sort | Chuo, Steven Ting-Yu |
collection | PubMed |
description | Extracellular vesicles (EVs) are lipid bilayer-enclosed nanoparticles released by cells. They range from 30 nm to several micrometers in diameter, and ferry biological cargos such as proteins, lipids, RNAs and DNAs for local and distant intercellular communications. EVs have since been found to play a role in development, as well as in diseases including cancers. To elucidate the roles of EVs, researchers have established different methods to visualize and study their spatiotemporal properties. However, since EV are nanometer-sized, imaging them demands a full understanding of each labeling strategy to ensure accurate monitoring. This review covers current and emerging strategies for EV imaging for prospective studies. |
format | Online Article Text |
id | pubmed-6304785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63047852019-01-02 Imaging extracellular vesicles: current and emerging methods Chuo, Steven Ting-Yu Chien, Jasper Che-Yung Lai, Charles Pin-Kuang J Biomed Sci Review Extracellular vesicles (EVs) are lipid bilayer-enclosed nanoparticles released by cells. They range from 30 nm to several micrometers in diameter, and ferry biological cargos such as proteins, lipids, RNAs and DNAs for local and distant intercellular communications. EVs have since been found to play a role in development, as well as in diseases including cancers. To elucidate the roles of EVs, researchers have established different methods to visualize and study their spatiotemporal properties. However, since EV are nanometer-sized, imaging them demands a full understanding of each labeling strategy to ensure accurate monitoring. This review covers current and emerging strategies for EV imaging for prospective studies. BioMed Central 2018-12-24 /pmc/articles/PMC6304785/ /pubmed/30580764 http://dx.doi.org/10.1186/s12929-018-0494-5 Text en © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Chuo, Steven Ting-Yu Chien, Jasper Che-Yung Lai, Charles Pin-Kuang Imaging extracellular vesicles: current and emerging methods |
title | Imaging extracellular vesicles: current and emerging methods |
title_full | Imaging extracellular vesicles: current and emerging methods |
title_fullStr | Imaging extracellular vesicles: current and emerging methods |
title_full_unstemmed | Imaging extracellular vesicles: current and emerging methods |
title_short | Imaging extracellular vesicles: current and emerging methods |
title_sort | imaging extracellular vesicles: current and emerging methods |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304785/ https://www.ncbi.nlm.nih.gov/pubmed/30580764 http://dx.doi.org/10.1186/s12929-018-0494-5 |
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