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Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients

The main aim of this study was to investigate the putative association among the presence of prostate cancer cells, defined as prostate osteoblast-like cells (POLCs), and showing the expression of typical morphological and molecular characteristics of osteoblasts, the development of bone metastasis...

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Autores principales: Scimeca, Manuel, Urbano, Nicoletta, Rita, Bonfiglio, Mapelli, Sarah Natalia, Catapano, Carlo Vittorio, Carbone, Giuseppina Maria, Ciuffa, Sara, Tavolozza, Mario, Schillaci, Orazio, Mauriello, Alessandro, Bonanno, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305022/
https://www.ncbi.nlm.nih.gov/pubmed/30627063
http://dx.doi.org/10.1155/2018/9840962
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author Scimeca, Manuel
Urbano, Nicoletta
Rita, Bonfiglio
Mapelli, Sarah Natalia
Catapano, Carlo Vittorio
Carbone, Giuseppina Maria
Ciuffa, Sara
Tavolozza, Mario
Schillaci, Orazio
Mauriello, Alessandro
Bonanno, Elena
author_facet Scimeca, Manuel
Urbano, Nicoletta
Rita, Bonfiglio
Mapelli, Sarah Natalia
Catapano, Carlo Vittorio
Carbone, Giuseppina Maria
Ciuffa, Sara
Tavolozza, Mario
Schillaci, Orazio
Mauriello, Alessandro
Bonanno, Elena
author_sort Scimeca, Manuel
collection PubMed
description The main aim of this study was to investigate the putative association among the presence of prostate cancer cells, defined as prostate osteoblast-like cells (POLCs), and showing the expression of typical morphological and molecular characteristics of osteoblasts, the development of bone metastasis within 5 years of diagnosis, and the uptake of 18F-choline evaluated by PET/CT analysis. To this end, prostate biopsies (n = 110) were collected comprising 44 benign lesions and 66 malignant lesions. Malignant lesions were further subdivided into two groups: biopsies from patients that had clinical evidence of bone metastasis (BM+, n = 23) and biopsies from patients that did not have clinical evidence of bone metastasis within 5 years (BM−, n = 43). Paraffin serial sections were obtained from each specimen to perform histological classifications and immunohistochemical (IHC) analysis. Small fragments of tissue were used to perform ultrastructural and microanalytical investigations. IHC demonstrated the expression of markers of epithelial-to-mesenchymal transition (VIM), bone mineralization, and osteoblastic differentiation (BMP-2, PTX-3, RUNX2, RANKL, and VDR) in prostate lesions characterized by the presence of calcium-phosphate microcalcifications and high metastatic potential. Ultrastructural studies revealed the presence of prostate cancer cells with osteoblast phenotype close to microcalcifications. Noteworthy, PET/CT analysis showed higher uptake of 18F-choline in BM+ lesions with high positivity (≥300/500 cells) for RUNX2 and/or RANKL immunostaining. Although these data require further investigations about the molecular mechanisms of POLCs generation and role in bone metastasis, our study can open new and interesting prospective in the management of prostate cancer patients. The presence of POLCs along with prostate microcalcifications may become negative prognostic markers of the occurrence of bone metastases.
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spelling pubmed-63050222019-01-09 Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients Scimeca, Manuel Urbano, Nicoletta Rita, Bonfiglio Mapelli, Sarah Natalia Catapano, Carlo Vittorio Carbone, Giuseppina Maria Ciuffa, Sara Tavolozza, Mario Schillaci, Orazio Mauriello, Alessandro Bonanno, Elena Contrast Media Mol Imaging Research Article The main aim of this study was to investigate the putative association among the presence of prostate cancer cells, defined as prostate osteoblast-like cells (POLCs), and showing the expression of typical morphological and molecular characteristics of osteoblasts, the development of bone metastasis within 5 years of diagnosis, and the uptake of 18F-choline evaluated by PET/CT analysis. To this end, prostate biopsies (n = 110) were collected comprising 44 benign lesions and 66 malignant lesions. Malignant lesions were further subdivided into two groups: biopsies from patients that had clinical evidence of bone metastasis (BM+, n = 23) and biopsies from patients that did not have clinical evidence of bone metastasis within 5 years (BM−, n = 43). Paraffin serial sections were obtained from each specimen to perform histological classifications and immunohistochemical (IHC) analysis. Small fragments of tissue were used to perform ultrastructural and microanalytical investigations. IHC demonstrated the expression of markers of epithelial-to-mesenchymal transition (VIM), bone mineralization, and osteoblastic differentiation (BMP-2, PTX-3, RUNX2, RANKL, and VDR) in prostate lesions characterized by the presence of calcium-phosphate microcalcifications and high metastatic potential. Ultrastructural studies revealed the presence of prostate cancer cells with osteoblast phenotype close to microcalcifications. Noteworthy, PET/CT analysis showed higher uptake of 18F-choline in BM+ lesions with high positivity (≥300/500 cells) for RUNX2 and/or RANKL immunostaining. Although these data require further investigations about the molecular mechanisms of POLCs generation and role in bone metastasis, our study can open new and interesting prospective in the management of prostate cancer patients. The presence of POLCs along with prostate microcalcifications may become negative prognostic markers of the occurrence of bone metastases. Hindawi 2018-12-09 /pmc/articles/PMC6305022/ /pubmed/30627063 http://dx.doi.org/10.1155/2018/9840962 Text en Copyright © 2018 Manuel Scimeca et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Scimeca, Manuel
Urbano, Nicoletta
Rita, Bonfiglio
Mapelli, Sarah Natalia
Catapano, Carlo Vittorio
Carbone, Giuseppina Maria
Ciuffa, Sara
Tavolozza, Mario
Schillaci, Orazio
Mauriello, Alessandro
Bonanno, Elena
Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients
title Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients
title_full Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients
title_fullStr Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients
title_full_unstemmed Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients
title_short Prostate Osteoblast-Like Cells: A Reliable Prognostic Marker of Bone Metastasis in Prostate Cancer Patients
title_sort prostate osteoblast-like cells: a reliable prognostic marker of bone metastasis in prostate cancer patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305022/
https://www.ncbi.nlm.nih.gov/pubmed/30627063
http://dx.doi.org/10.1155/2018/9840962
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