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Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars

MicroRNAs (miRNAs) play an important role in plant resistance to pathogen infections. However, little is known about the role of miRNAs in banana Fusarium wilt, which is the most economically devastating disease in banana production. In the present study, we identified and characterized a total of 1...

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Autores principales: Song, Shun, Xu, Yi, Huang, Dongmei, Ashraf, Muhammad Aleem, Li, Jingyang, Hu, Wei, Jin, Zhiqiang, Zeng, Changying, Tang, Fenling, Xu, Biyu, Zeng, Huicai, Li, Yujia, Xie, Jianghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305118/
https://www.ncbi.nlm.nih.gov/pubmed/30595993
http://dx.doi.org/10.7717/peerj.6209
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author Song, Shun
Xu, Yi
Huang, Dongmei
Ashraf, Muhammad Aleem
Li, Jingyang
Hu, Wei
Jin, Zhiqiang
Zeng, Changying
Tang, Fenling
Xu, Biyu
Zeng, Huicai
Li, Yujia
Xie, Jianghui
author_facet Song, Shun
Xu, Yi
Huang, Dongmei
Ashraf, Muhammad Aleem
Li, Jingyang
Hu, Wei
Jin, Zhiqiang
Zeng, Changying
Tang, Fenling
Xu, Biyu
Zeng, Huicai
Li, Yujia
Xie, Jianghui
author_sort Song, Shun
collection PubMed
description MicroRNAs (miRNAs) play an important role in plant resistance to pathogen infections. However, little is known about the role of miRNAs in banana Fusarium wilt, which is the most economically devastating disease in banana production. In the present study, we identified and characterized a total of 18 miR169 family members in banana (Musa acuminata L.) based on small RNA sequencing. The banana miR169 family clustered into two groups based on miRNA evolutionary analysis. Multiple sequence alignment indicated a high degree of sequence conservation in miRNA169 family members across 28 plant species. Computational target prediction algorithms were used to identify 25 targets of miR169 family members in banana. These targets were enriched in various metabolic pathways that include the following molecules: glycine, serine, threonine, pentose, glycerolipids, nucleotide sugars, starch, and sucrose. Through miRNA transcriptomic analysis, we found that ma-miR169a and ma-miR169b displayed high expression levels, whereas the other 16 ma-miR169 members exhibited low expression in the HG and Baxi banana cultivars. Further experiments indicate that there were negative relationships between ma-miR169a, ma-miR169b and their targets basing on their expression levels to Foc4 (Fusarium oxysporum f. sp. cubense tropical race 4) infection in resistant cultivars. But they were low expressed in susceptive cultivars. These results suggested that the expression levels of ma-miR169a and ma-miR169b were consistent with the resistance degree of the banana cultivars to Foc4. The analysis presented here constitutes a starting point to understand ma-miR169-mediated Fusarium wilt resistance at the transcriptional level in banana and predicts possible candidate targets for the genetic improvement of banana resistance to Foc4.
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spelling pubmed-63051182018-12-28 Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars Song, Shun Xu, Yi Huang, Dongmei Ashraf, Muhammad Aleem Li, Jingyang Hu, Wei Jin, Zhiqiang Zeng, Changying Tang, Fenling Xu, Biyu Zeng, Huicai Li, Yujia Xie, Jianghui PeerJ Microbiology MicroRNAs (miRNAs) play an important role in plant resistance to pathogen infections. However, little is known about the role of miRNAs in banana Fusarium wilt, which is the most economically devastating disease in banana production. In the present study, we identified and characterized a total of 18 miR169 family members in banana (Musa acuminata L.) based on small RNA sequencing. The banana miR169 family clustered into two groups based on miRNA evolutionary analysis. Multiple sequence alignment indicated a high degree of sequence conservation in miRNA169 family members across 28 plant species. Computational target prediction algorithms were used to identify 25 targets of miR169 family members in banana. These targets were enriched in various metabolic pathways that include the following molecules: glycine, serine, threonine, pentose, glycerolipids, nucleotide sugars, starch, and sucrose. Through miRNA transcriptomic analysis, we found that ma-miR169a and ma-miR169b displayed high expression levels, whereas the other 16 ma-miR169 members exhibited low expression in the HG and Baxi banana cultivars. Further experiments indicate that there were negative relationships between ma-miR169a, ma-miR169b and their targets basing on their expression levels to Foc4 (Fusarium oxysporum f. sp. cubense tropical race 4) infection in resistant cultivars. But they were low expressed in susceptive cultivars. These results suggested that the expression levels of ma-miR169a and ma-miR169b were consistent with the resistance degree of the banana cultivars to Foc4. The analysis presented here constitutes a starting point to understand ma-miR169-mediated Fusarium wilt resistance at the transcriptional level in banana and predicts possible candidate targets for the genetic improvement of banana resistance to Foc4. PeerJ Inc. 2018-12-21 /pmc/articles/PMC6305118/ /pubmed/30595993 http://dx.doi.org/10.7717/peerj.6209 Text en © 2018 Song et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Song, Shun
Xu, Yi
Huang, Dongmei
Ashraf, Muhammad Aleem
Li, Jingyang
Hu, Wei
Jin, Zhiqiang
Zeng, Changying
Tang, Fenling
Xu, Biyu
Zeng, Huicai
Li, Yujia
Xie, Jianghui
Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
title Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
title_full Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
title_fullStr Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
title_full_unstemmed Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
title_short Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
title_sort identification and characterization of mirna169 family members in banana (musa acuminata l.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305118/
https://www.ncbi.nlm.nih.gov/pubmed/30595993
http://dx.doi.org/10.7717/peerj.6209
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