Cargando…

A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes

Tomato spotted wilt virus (TSWV; Genus Orthotospovirus: Family Tospoviridae) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were coll...

Descripción completa

Detalles Bibliográficos
Autores principales: Abadkhah, Mahsa, Koolivand, Davoud, Eini, Omid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305171/
https://www.ncbi.nlm.nih.gov/pubmed/30588225
http://dx.doi.org/10.5423/PPJ.OA.04.2018.0062
_version_ 1783382506038362112
author Abadkhah, Mahsa
Koolivand, Davoud
Eini, Omid
author_facet Abadkhah, Mahsa
Koolivand, Davoud
Eini, Omid
author_sort Abadkhah, Mahsa
collection PubMed
description Tomato spotted wilt virus (TSWV; Genus Orthotospovirus: Family Tospoviridae) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RT-PCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor evolutionary differences exist amongst the isolates. The phylogenetic tree grouped all isolates six clades based on N and NSs genes. Phylogenetic analysis showed that the Iranian isolates were composed a new distinct clade based on a part of polymerase, N and NSs genes. To our knowledge, this is the first detailed study on molecular characterization and genetic diversity of TSWV isolates from tomato in Iran that could be known as new clade of TSWV isolates.
format Online
Article
Text
id pubmed-6305171
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Korean Society of Plant Pathology
record_format MEDLINE/PubMed
spelling pubmed-63051712018-12-26 A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes Abadkhah, Mahsa Koolivand, Davoud Eini, Omid Plant Pathol J Research Article Tomato spotted wilt virus (TSWV; Genus Orthotospovirus: Family Tospoviridae) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RT-PCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor evolutionary differences exist amongst the isolates. The phylogenetic tree grouped all isolates six clades based on N and NSs genes. Phylogenetic analysis showed that the Iranian isolates were composed a new distinct clade based on a part of polymerase, N and NSs genes. To our knowledge, this is the first detailed study on molecular characterization and genetic diversity of TSWV isolates from tomato in Iran that could be known as new clade of TSWV isolates. Korean Society of Plant Pathology 2018-12 2018-12-01 /pmc/articles/PMC6305171/ /pubmed/30588225 http://dx.doi.org/10.5423/PPJ.OA.04.2018.0062 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abadkhah, Mahsa
Koolivand, Davoud
Eini, Omid
A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
title A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
title_full A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
title_fullStr A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
title_full_unstemmed A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
title_short A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
title_sort new distinct clade for iranian tomato spotted wilt virus isolates based on the polymerase, nucleocapsid, and non-structural genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305171/
https://www.ncbi.nlm.nih.gov/pubmed/30588225
http://dx.doi.org/10.5423/PPJ.OA.04.2018.0062
work_keys_str_mv AT abadkhahmahsa anewdistinctcladeforiraniantomatospottedwiltvirusisolatesbasedonthepolymerasenucleocapsidandnonstructuralgenes
AT koolivanddavoud anewdistinctcladeforiraniantomatospottedwiltvirusisolatesbasedonthepolymerasenucleocapsidandnonstructuralgenes
AT einiomid anewdistinctcladeforiraniantomatospottedwiltvirusisolatesbasedonthepolymerasenucleocapsidandnonstructuralgenes
AT abadkhahmahsa newdistinctcladeforiraniantomatospottedwiltvirusisolatesbasedonthepolymerasenucleocapsidandnonstructuralgenes
AT koolivanddavoud newdistinctcladeforiraniantomatospottedwiltvirusisolatesbasedonthepolymerasenucleocapsidandnonstructuralgenes
AT einiomid newdistinctcladeforiraniantomatospottedwiltvirusisolatesbasedonthepolymerasenucleocapsidandnonstructuralgenes