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Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reve...

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Detalles Bibliográficos
Autores principales: Kim, Nam-Yeon, Oh, Jonghee, Lee, Su-Heon, Kim, Hongsup, Moon, Jae Sun, Jeong, Rae-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305176/
https://www.ncbi.nlm.nih.gov/pubmed/30588230
http://dx.doi.org/10.5423/PPJ.NT.06.2018.0108
Descripción
Sumario:Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories.