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Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reve...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Plant Pathology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305176/ https://www.ncbi.nlm.nih.gov/pubmed/30588230 http://dx.doi.org/10.5423/PPJ.NT.06.2018.0108 |
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author | Kim, Nam-Yeon Oh, Jonghee Lee, Su-Heon Kim, Hongsup Moon, Jae Sun Jeong, Rae-Dong |
author_facet | Kim, Nam-Yeon Oh, Jonghee Lee, Su-Heon Kim, Hongsup Moon, Jae Sun Jeong, Rae-Dong |
author_sort | Kim, Nam-Yeon |
collection | PubMed |
description | Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories. |
format | Online Article Text |
id | pubmed-6305176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-63051762018-12-26 Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification Kim, Nam-Yeon Oh, Jonghee Lee, Su-Heon Kim, Hongsup Moon, Jae Sun Jeong, Rae-Dong Plant Pathol J Note Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories. Korean Society of Plant Pathology 2018-12 2018-12-01 /pmc/articles/PMC6305176/ /pubmed/30588230 http://dx.doi.org/10.5423/PPJ.NT.06.2018.0108 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Note Kim, Nam-Yeon Oh, Jonghee Lee, Su-Heon Kim, Hongsup Moon, Jae Sun Jeong, Rae-Dong Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title | Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_full | Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_fullStr | Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_full_unstemmed | Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_short | Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification |
title_sort | rapid and specific detection of apple stem grooving virus by reverse transcription-recombinase polymerase amplification |
topic | Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305176/ https://www.ncbi.nlm.nih.gov/pubmed/30588230 http://dx.doi.org/10.5423/PPJ.NT.06.2018.0108 |
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