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DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism

Soybean seeds contain higher concentrations of oil (triacylglycerol) and fatty acids than do cereal crop seeds, and the oxidation of these biomolecules during seed storage significantly shortens seed longevity and decreases germination ability. Here, we report that diethyl aminoethyl hexanoate (DA-6...

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Autores principales: Zhou, Wenguan, Chen, Feng, Zhao, Sihua, Yang, Caiqiong, Meng, Yongjie, Shuai, Haiwei, Luo, Xiaofeng, Dai, Yujia, Yin, Han, Du, Junbo, Liu, Jiang, Fan, Gaoqiong, Liu, Weiguo, Yang, Wenyu, Shu, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305204/
https://www.ncbi.nlm.nih.gov/pubmed/29982626
http://dx.doi.org/10.1093/jxb/ery247
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author Zhou, Wenguan
Chen, Feng
Zhao, Sihua
Yang, Caiqiong
Meng, Yongjie
Shuai, Haiwei
Luo, Xiaofeng
Dai, Yujia
Yin, Han
Du, Junbo
Liu, Jiang
Fan, Gaoqiong
Liu, Weiguo
Yang, Wenyu
Shu, Kai
author_facet Zhou, Wenguan
Chen, Feng
Zhao, Sihua
Yang, Caiqiong
Meng, Yongjie
Shuai, Haiwei
Luo, Xiaofeng
Dai, Yujia
Yin, Han
Du, Junbo
Liu, Jiang
Fan, Gaoqiong
Liu, Weiguo
Yang, Wenyu
Shu, Kai
author_sort Zhou, Wenguan
collection PubMed
description Soybean seeds contain higher concentrations of oil (triacylglycerol) and fatty acids than do cereal crop seeds, and the oxidation of these biomolecules during seed storage significantly shortens seed longevity and decreases germination ability. Here, we report that diethyl aminoethyl hexanoate (DA-6), a plant growth regulator, increases germination and seedling establishment from aged soybean seeds by increasing fatty acid metabolism and glycometabolism. Phenotypic analysis showed that DA-6 treatment markedly promoted germination and seedling establishment from naturally and artificially aged soybean seeds. Further analysis revealed that DA-6 increased the concentrations of soluble sugars during imbibition of aged soybean seeds. Consistently, the concentrations of several different fatty acids in DA-6-treated aged seeds were higher than those in untreated aged seeds. Subsequently, quantitative PCR analysis indicated that DA-6 induced the transcription of several key genes involved in the hydrolysis of triacylglycerol to sugars in aged soybean seeds. Furthermore, the activity of invertase in aged seeds, which catalyzes the hydrolysis of sucrose to form fructose and glucose, increased following DA-6 treatment. Taken together, DA-6 promotes germination and seedling establishment from aged soybean seeds by enhancing the hydrolysis of triacylglycerol and the conversion of fatty acids to sugars.
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spelling pubmed-63052042019-01-07 DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism Zhou, Wenguan Chen, Feng Zhao, Sihua Yang, Caiqiong Meng, Yongjie Shuai, Haiwei Luo, Xiaofeng Dai, Yujia Yin, Han Du, Junbo Liu, Jiang Fan, Gaoqiong Liu, Weiguo Yang, Wenyu Shu, Kai J Exp Bot Research Papers Soybean seeds contain higher concentrations of oil (triacylglycerol) and fatty acids than do cereal crop seeds, and the oxidation of these biomolecules during seed storage significantly shortens seed longevity and decreases germination ability. Here, we report that diethyl aminoethyl hexanoate (DA-6), a plant growth regulator, increases germination and seedling establishment from aged soybean seeds by increasing fatty acid metabolism and glycometabolism. Phenotypic analysis showed that DA-6 treatment markedly promoted germination and seedling establishment from naturally and artificially aged soybean seeds. Further analysis revealed that DA-6 increased the concentrations of soluble sugars during imbibition of aged soybean seeds. Consistently, the concentrations of several different fatty acids in DA-6-treated aged seeds were higher than those in untreated aged seeds. Subsequently, quantitative PCR analysis indicated that DA-6 induced the transcription of several key genes involved in the hydrolysis of triacylglycerol to sugars in aged soybean seeds. Furthermore, the activity of invertase in aged seeds, which catalyzes the hydrolysis of sucrose to form fructose and glucose, increased following DA-6 treatment. Taken together, DA-6 promotes germination and seedling establishment from aged soybean seeds by enhancing the hydrolysis of triacylglycerol and the conversion of fatty acids to sugars. Oxford University Press 2019-01-01 2018-07-03 /pmc/articles/PMC6305204/ /pubmed/29982626 http://dx.doi.org/10.1093/jxb/ery247 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Zhou, Wenguan
Chen, Feng
Zhao, Sihua
Yang, Caiqiong
Meng, Yongjie
Shuai, Haiwei
Luo, Xiaofeng
Dai, Yujia
Yin, Han
Du, Junbo
Liu, Jiang
Fan, Gaoqiong
Liu, Weiguo
Yang, Wenyu
Shu, Kai
DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism
title DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism
title_full DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism
title_fullStr DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism
title_full_unstemmed DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism
title_short DA-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism
title_sort da-6 promotes germination and seedling establishment from aged soybean seeds by mediating fatty acid metabolism and glycometabolism
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305204/
https://www.ncbi.nlm.nih.gov/pubmed/29982626
http://dx.doi.org/10.1093/jxb/ery247
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