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Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats

Aging is associated with morphological, physiological and metabolic changes, leading to multiorgan degenerative pathologies, such as cognitive function decline. It has been suggested that memory loss also involves a decrease in neurotrophic factors, including brain-derived neurotrophic factor (BDNF)...

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Autores principales: Romo-Araiza, Alejandra, Gutiérrez-Salmeán, Gabriela, Galván, Emilio J., Hernández-Frausto, Melissa, Herrera-López, Gabriel, Romo-Parra, Hector, García-Contreras, Valentina, Fernández-Presas, Ana María, Jasso-Chávez, Ricardo, Borlongan, Cesar V., Ibarra, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305305/
https://www.ncbi.nlm.nih.gov/pubmed/30618722
http://dx.doi.org/10.3389/fnagi.2018.00416
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author Romo-Araiza, Alejandra
Gutiérrez-Salmeán, Gabriela
Galván, Emilio J.
Hernández-Frausto, Melissa
Herrera-López, Gabriel
Romo-Parra, Hector
García-Contreras, Valentina
Fernández-Presas, Ana María
Jasso-Chávez, Ricardo
Borlongan, Cesar V.
Ibarra, Antonio
author_facet Romo-Araiza, Alejandra
Gutiérrez-Salmeán, Gabriela
Galván, Emilio J.
Hernández-Frausto, Melissa
Herrera-López, Gabriel
Romo-Parra, Hector
García-Contreras, Valentina
Fernández-Presas, Ana María
Jasso-Chávez, Ricardo
Borlongan, Cesar V.
Ibarra, Antonio
author_sort Romo-Araiza, Alejandra
collection PubMed
description Aging is associated with morphological, physiological and metabolic changes, leading to multiorgan degenerative pathologies, such as cognitive function decline. It has been suggested that memory loss also involves a decrease in neurotrophic factors, including brain-derived neurotrophic factor (BDNF). In recent years, microbiota has been proposed as an essential player in brain development, as it is believed to activate BDNF secretion through butyrate production. Thus, microbiota modulation by supplementation with probiotics and prebiotics may impact cognitive decline. This study aimed to evaluate the effects of probiotics and prebiotics supplementation on the memory of middle-aged rats. Sprague-Dawley male rats were randomized in four groups (n = 13 per group): control (water), probiotic (E. faecium), prebiotic (agave inulin), symbiotic (E. faecium + inulin), which were administered for 5 weeks by oral gavage. Spatial and associative memory was analyzed using the Morris Water Maze (MWM) and Pavlovian autoshaping tests, respectively. Hippocampus was obtained to analyze cytokines [interleukin (IL-1β) and tumor necrosis factor (TNF-α)], BDNF and γ-aminobutyric acid (GABA) by enzyme-linked immunosorbent assay (ELISA). Butyrate concentrations were also evaluated in feces. The symbiotic group showed a significantly better performance in MWM (p < 0.01), but not in Pavlovian autoshaping test. It also showed significantly lower concentrations of pro-inflammatory cytokines (p < 0.01) and the reduction in IL-1β correlated with a better performance of the symbiotic group in MWM (p < 0.05). Symbiotic group also showed the highest BDNF and butyrate levels (p < 0.0001). Finally, we compared the electrophysiological responses of control (n = 8) and symbiotic (n = 8) groups. Passive properties of CA1 pyramidal cells (PCs) exhibited changes in response to the symbiotic treatment. Likewise, this group showed an increase in the N-methyl-D-aspartate receptor (NMDA)/AMPA ratio and exhibited robust long-term potentiation (LTP; p < 0.01). Integrated results suggest that symbiotics could improve age-related impaired memory.
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spelling pubmed-63053052019-01-07 Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats Romo-Araiza, Alejandra Gutiérrez-Salmeán, Gabriela Galván, Emilio J. Hernández-Frausto, Melissa Herrera-López, Gabriel Romo-Parra, Hector García-Contreras, Valentina Fernández-Presas, Ana María Jasso-Chávez, Ricardo Borlongan, Cesar V. Ibarra, Antonio Front Aging Neurosci Neuroscience Aging is associated with morphological, physiological and metabolic changes, leading to multiorgan degenerative pathologies, such as cognitive function decline. It has been suggested that memory loss also involves a decrease in neurotrophic factors, including brain-derived neurotrophic factor (BDNF). In recent years, microbiota has been proposed as an essential player in brain development, as it is believed to activate BDNF secretion through butyrate production. Thus, microbiota modulation by supplementation with probiotics and prebiotics may impact cognitive decline. This study aimed to evaluate the effects of probiotics and prebiotics supplementation on the memory of middle-aged rats. Sprague-Dawley male rats were randomized in four groups (n = 13 per group): control (water), probiotic (E. faecium), prebiotic (agave inulin), symbiotic (E. faecium + inulin), which were administered for 5 weeks by oral gavage. Spatial and associative memory was analyzed using the Morris Water Maze (MWM) and Pavlovian autoshaping tests, respectively. Hippocampus was obtained to analyze cytokines [interleukin (IL-1β) and tumor necrosis factor (TNF-α)], BDNF and γ-aminobutyric acid (GABA) by enzyme-linked immunosorbent assay (ELISA). Butyrate concentrations were also evaluated in feces. The symbiotic group showed a significantly better performance in MWM (p < 0.01), but not in Pavlovian autoshaping test. It also showed significantly lower concentrations of pro-inflammatory cytokines (p < 0.01) and the reduction in IL-1β correlated with a better performance of the symbiotic group in MWM (p < 0.05). Symbiotic group also showed the highest BDNF and butyrate levels (p < 0.0001). Finally, we compared the electrophysiological responses of control (n = 8) and symbiotic (n = 8) groups. Passive properties of CA1 pyramidal cells (PCs) exhibited changes in response to the symbiotic treatment. Likewise, this group showed an increase in the N-methyl-D-aspartate receptor (NMDA)/AMPA ratio and exhibited robust long-term potentiation (LTP; p < 0.01). Integrated results suggest that symbiotics could improve age-related impaired memory. Frontiers Media S.A. 2018-12-18 /pmc/articles/PMC6305305/ /pubmed/30618722 http://dx.doi.org/10.3389/fnagi.2018.00416 Text en Copyright © 2018 Romo-Araiza, Gutiérrez-Salmeán, Galván, Hernández-Frausto, Herrera-López, Romo-Parra, García-Contreras, Fernández-Presas, Jasso-Chávez, Borlongan and Ibarra. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Romo-Araiza, Alejandra
Gutiérrez-Salmeán, Gabriela
Galván, Emilio J.
Hernández-Frausto, Melissa
Herrera-López, Gabriel
Romo-Parra, Hector
García-Contreras, Valentina
Fernández-Presas, Ana María
Jasso-Chávez, Ricardo
Borlongan, Cesar V.
Ibarra, Antonio
Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats
title Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats
title_full Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats
title_fullStr Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats
title_full_unstemmed Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats
title_short Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats
title_sort probiotics and prebiotics as a therapeutic strategy to improve memory in a model of middle-aged rats
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305305/
https://www.ncbi.nlm.nih.gov/pubmed/30618722
http://dx.doi.org/10.3389/fnagi.2018.00416
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