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Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets

Weaned piglets are vulnerable to nutritional, physiological, and psychological stressors, leading to abrupt taxonomic and functional shifts in the intestinal microbiome. In this study, an integrated approach combination of 16S rDNA gene sequencing and the mass spectrometry-based metabolomics techniq...

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Autores principales: Li, Yuan, Guo, Yong, Wen, Zhengshun, Jiang, Xuemei, Ma, Xin, Han, Xinyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305375/
https://www.ncbi.nlm.nih.gov/pubmed/30584255
http://dx.doi.org/10.1038/s41598-018-33649-8
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author Li, Yuan
Guo, Yong
Wen, Zhengshun
Jiang, Xuemei
Ma, Xin
Han, Xinyan
author_facet Li, Yuan
Guo, Yong
Wen, Zhengshun
Jiang, Xuemei
Ma, Xin
Han, Xinyan
author_sort Li, Yuan
collection PubMed
description Weaned piglets are vulnerable to nutritional, physiological, and psychological stressors, leading to abrupt taxonomic and functional shifts in the intestinal microbiome. In this study, an integrated approach combination of 16S rDNA gene sequencing and the mass spectrometry-based metabolomics techniques was used to investigate the effects of weaning stress on intestinal microbial composition and its metabolic profiles of piglets. Three litters of suckling piglets with same parity were chosen. The samples of colonic contents were collected from each selected piglets (weaned day, 3 days after weaned) for microbial and metabolomics analysis. The results showed that Lachnospiraceae, Negativicutes, Selenomonadales, Campylobacterales and other 15 species increased after weaning, while Porphyromonadaceace, Alloprevotella, Barnesiella and Oscillibacter decreased. Based on the function profiles prediction and metabolomic analysis, five key metabolic pathways including Phenylalanine metabolism, Citrate cycle (TCA cycle), Glycolysis or Gluconeogenesis, Propanoate metabolism, Nicotinate and nicotinamide metabolism might be the relevant pathways involved in weaning stress-induced gut microbiota dysbiosis. Taken together, these results indicated that weaning stress not only changed microbial composition and function but altered the microbial metabolic profiles in the intestine, which might provide a new insight in alleviating weaning stress and facilitating disease prevention during the period of weaning in piglets.
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spelling pubmed-63053752018-12-31 Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets Li, Yuan Guo, Yong Wen, Zhengshun Jiang, Xuemei Ma, Xin Han, Xinyan Sci Rep Article Weaned piglets are vulnerable to nutritional, physiological, and psychological stressors, leading to abrupt taxonomic and functional shifts in the intestinal microbiome. In this study, an integrated approach combination of 16S rDNA gene sequencing and the mass spectrometry-based metabolomics techniques was used to investigate the effects of weaning stress on intestinal microbial composition and its metabolic profiles of piglets. Three litters of suckling piglets with same parity were chosen. The samples of colonic contents were collected from each selected piglets (weaned day, 3 days after weaned) for microbial and metabolomics analysis. The results showed that Lachnospiraceae, Negativicutes, Selenomonadales, Campylobacterales and other 15 species increased after weaning, while Porphyromonadaceace, Alloprevotella, Barnesiella and Oscillibacter decreased. Based on the function profiles prediction and metabolomic analysis, five key metabolic pathways including Phenylalanine metabolism, Citrate cycle (TCA cycle), Glycolysis or Gluconeogenesis, Propanoate metabolism, Nicotinate and nicotinamide metabolism might be the relevant pathways involved in weaning stress-induced gut microbiota dysbiosis. Taken together, these results indicated that weaning stress not only changed microbial composition and function but altered the microbial metabolic profiles in the intestine, which might provide a new insight in alleviating weaning stress and facilitating disease prevention during the period of weaning in piglets. Nature Publishing Group UK 2018-12-24 /pmc/articles/PMC6305375/ /pubmed/30584255 http://dx.doi.org/10.1038/s41598-018-33649-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Yuan
Guo, Yong
Wen, Zhengshun
Jiang, Xuemei
Ma, Xin
Han, Xinyan
Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets
title Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets
title_full Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets
title_fullStr Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets
title_full_unstemmed Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets
title_short Weaning Stress Perturbs Gut Microbiome and Its Metabolic Profile in Piglets
title_sort weaning stress perturbs gut microbiome and its metabolic profile in piglets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305375/
https://www.ncbi.nlm.nih.gov/pubmed/30584255
http://dx.doi.org/10.1038/s41598-018-33649-8
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