Cargando…

Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride

Aging is a complex process involving declines in various cellular and physical functionalities, including regenerative ability. Telomere maintenance is thought to be necessary for regeneration, and telomere attrition is one mechanism that contributes to aging. However, it is unclear if aging affects...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Chi-Fan, Sung, Tzu-Ling, Chen, Liuh-Yow, Chen, Jiun-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305377/
https://www.ncbi.nlm.nih.gov/pubmed/30584242
http://dx.doi.org/10.1038/s41598-018-36396-y
_version_ 1783382550046048256
author Chen, Chi-Fan
Sung, Tzu-Ling
Chen, Liuh-Yow
Chen, Jiun-Hong
author_facet Chen, Chi-Fan
Sung, Tzu-Ling
Chen, Liuh-Yow
Chen, Jiun-Hong
author_sort Chen, Chi-Fan
collection PubMed
description Aging is a complex process involving declines in various cellular and physical functionalities, including regenerative ability. Telomere maintenance is thought to be necessary for regeneration, and telomere attrition is one mechanism that contributes to aging. However, it is unclear if aging affects regeneration owing to deterioration of telomeric maintenance. We introduce Aeolosoma viride—a freshwater annelid with strong regenerative abilities—as a new model for studying the effects of aging on telomere functions and regeneration. We show that the anterior regenerative ability of A. viride declines with age. We characterized the A. viride telomere sequence as being composed of TTAGGG repeats and identifyied the telomerase gene Avi-tert. In adult A. viride, telomerase was constantly active and telomere lengths were similar among different body sections and stably maintained with age. Notably, we found that regeneration did not result in telomere shortening at regenerating sites. Moreover, transient up-regulation of Avi-tert expression and telomerase activity was observed at regenerating sites, which might promote telomere lengthening to counteract telomere erosion resulting from cell proliferation. Our study suggests that although aging affects A. viride regeneration independent of steady-state telomere length, timely regulation of telomerase functions is critical for the regeneration process in A. viride.
format Online
Article
Text
id pubmed-6305377
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-63053772018-12-31 Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride Chen, Chi-Fan Sung, Tzu-Ling Chen, Liuh-Yow Chen, Jiun-Hong Sci Rep Article Aging is a complex process involving declines in various cellular and physical functionalities, including regenerative ability. Telomere maintenance is thought to be necessary for regeneration, and telomere attrition is one mechanism that contributes to aging. However, it is unclear if aging affects regeneration owing to deterioration of telomeric maintenance. We introduce Aeolosoma viride—a freshwater annelid with strong regenerative abilities—as a new model for studying the effects of aging on telomere functions and regeneration. We show that the anterior regenerative ability of A. viride declines with age. We characterized the A. viride telomere sequence as being composed of TTAGGG repeats and identifyied the telomerase gene Avi-tert. In adult A. viride, telomerase was constantly active and telomere lengths were similar among different body sections and stably maintained with age. Notably, we found that regeneration did not result in telomere shortening at regenerating sites. Moreover, transient up-regulation of Avi-tert expression and telomerase activity was observed at regenerating sites, which might promote telomere lengthening to counteract telomere erosion resulting from cell proliferation. Our study suggests that although aging affects A. viride regeneration independent of steady-state telomere length, timely regulation of telomerase functions is critical for the regeneration process in A. viride. Nature Publishing Group UK 2018-12-24 /pmc/articles/PMC6305377/ /pubmed/30584242 http://dx.doi.org/10.1038/s41598-018-36396-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chen, Chi-Fan
Sung, Tzu-Ling
Chen, Liuh-Yow
Chen, Jiun-Hong
Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride
title Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride
title_full Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride
title_fullStr Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride
title_full_unstemmed Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride
title_short Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride
title_sort telomere maintenance during anterior regeneration and aging in the freshwater annelid aeolosoma viride
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305377/
https://www.ncbi.nlm.nih.gov/pubmed/30584242
http://dx.doi.org/10.1038/s41598-018-36396-y
work_keys_str_mv AT chenchifan telomeremaintenanceduringanteriorregenerationandaginginthefreshwaterannelidaeolosomaviride
AT sungtzuling telomeremaintenanceduringanteriorregenerationandaginginthefreshwaterannelidaeolosomaviride
AT chenliuhyow telomeremaintenanceduringanteriorregenerationandaginginthefreshwaterannelidaeolosomaviride
AT chenjiunhong telomeremaintenanceduringanteriorregenerationandaginginthefreshwaterannelidaeolosomaviride