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Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer

Oxymatrine (OMT) has shown broad antitumor activities for the treatment of several types of cancers. However, little is known about its effect on anti-tumor immunity. Combination therapy is a potentially promising strategy of cancer to enhance anticancer activity, overcome drug resistance, and lower...

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Autores principales: Ye, Jin, Zou, Man-Man, Li, Pei, Lin, Xi-Jun, Jiang, Qi-Wei, Yang, Yang, Huang, Jia-Rong, Yuan, Meng-Ling, Xing, Zi-Hao, Wei, Meng-Ning, Li, Yao, Shi, Zhi, Liu, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305450/
https://www.ncbi.nlm.nih.gov/pubmed/30619765
http://dx.doi.org/10.3389/fonc.2018.00631
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author Ye, Jin
Zou, Man-Man
Li, Pei
Lin, Xi-Jun
Jiang, Qi-Wei
Yang, Yang
Huang, Jia-Rong
Yuan, Meng-Ling
Xing, Zi-Hao
Wei, Meng-Ning
Li, Yao
Shi, Zhi
Liu, Hui
author_facet Ye, Jin
Zou, Man-Man
Li, Pei
Lin, Xi-Jun
Jiang, Qi-Wei
Yang, Yang
Huang, Jia-Rong
Yuan, Meng-Ling
Xing, Zi-Hao
Wei, Meng-Ning
Li, Yao
Shi, Zhi
Liu, Hui
author_sort Ye, Jin
collection PubMed
description Oxymatrine (OMT) has shown broad antitumor activities for the treatment of several types of cancers. However, little is known about its effect on anti-tumor immunity. Combination therapy is a potentially promising strategy of cancer to enhance anticancer activity, overcome drug resistance, and lower treatment failure rate. In the present study, we demonstrated that the combination of OMT with cisplatin (DDP) synergistically inhibited non-small cell lung cancer (NSCLC) cells growth when co-cultured with peripheral blood mononuclear cells in vitro. Furthermore, the combination of OMT with DDP significantly inhibited the growth of Lewis lung cancer (LLC) mouse xenograft tumors. Flow cytometry analysis revealed that OMT and DDP synergistically increase the CD8(+)/ regulatory T cells ratio and enhanced more CD8(+) T cells secreted cytokines of IFN-γ, TNF-α, and IL-2 in vivo. Mechanistically, upregulation of miR-155 and downregulation of suppressor of cytokine signaling-1 (SOCS1) were confirmed as a target signaling pathway to positively regulate the anti-tumor response of CD8(+) T cells. Overall, OMT in combination with DDP showed outstanding synergistic anti-tumor immunity, suggesting that this beneficial combination may offer a potential immunotherapy for NSCLC patients.
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spelling pubmed-63054502019-01-07 Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer Ye, Jin Zou, Man-Man Li, Pei Lin, Xi-Jun Jiang, Qi-Wei Yang, Yang Huang, Jia-Rong Yuan, Meng-Ling Xing, Zi-Hao Wei, Meng-Ning Li, Yao Shi, Zhi Liu, Hui Front Oncol Oncology Oxymatrine (OMT) has shown broad antitumor activities for the treatment of several types of cancers. However, little is known about its effect on anti-tumor immunity. Combination therapy is a potentially promising strategy of cancer to enhance anticancer activity, overcome drug resistance, and lower treatment failure rate. In the present study, we demonstrated that the combination of OMT with cisplatin (DDP) synergistically inhibited non-small cell lung cancer (NSCLC) cells growth when co-cultured with peripheral blood mononuclear cells in vitro. Furthermore, the combination of OMT with DDP significantly inhibited the growth of Lewis lung cancer (LLC) mouse xenograft tumors. Flow cytometry analysis revealed that OMT and DDP synergistically increase the CD8(+)/ regulatory T cells ratio and enhanced more CD8(+) T cells secreted cytokines of IFN-γ, TNF-α, and IL-2 in vivo. Mechanistically, upregulation of miR-155 and downregulation of suppressor of cytokine signaling-1 (SOCS1) were confirmed as a target signaling pathway to positively regulate the anti-tumor response of CD8(+) T cells. Overall, OMT in combination with DDP showed outstanding synergistic anti-tumor immunity, suggesting that this beneficial combination may offer a potential immunotherapy for NSCLC patients. Frontiers Media S.A. 2018-12-18 /pmc/articles/PMC6305450/ /pubmed/30619765 http://dx.doi.org/10.3389/fonc.2018.00631 Text en Copyright © 2018 Ye, Zou, Li, Lin, Jiang, Yang, Huang, Yuan, Xing, Wei, Li, Shi and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Ye, Jin
Zou, Man-Man
Li, Pei
Lin, Xi-Jun
Jiang, Qi-Wei
Yang, Yang
Huang, Jia-Rong
Yuan, Meng-Ling
Xing, Zi-Hao
Wei, Meng-Ning
Li, Yao
Shi, Zhi
Liu, Hui
Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer
title Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer
title_full Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer
title_fullStr Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer
title_full_unstemmed Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer
title_short Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8(+) T Cells in Non-small Cell Lung Cancer
title_sort oxymatrine and cisplatin synergistically enhance anti-tumor immunity of cd8(+) t cells in non-small cell lung cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305450/
https://www.ncbi.nlm.nih.gov/pubmed/30619765
http://dx.doi.org/10.3389/fonc.2018.00631
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