Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs

Recently, we reported that follicle-sinus complexes (FSCs) in the muzzle skin are useful for postmortem diagnosis of rabid dogs. Here, we compared the sensitivity and specificity of detecting the viral antigen in the brain and FSCs of 226 suspected rabid dogs, and assessed whether the FSC harbored t...

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Autores principales: SHIWA, Nozomi, NAKAJIMA, Chikage, KIMITSUKI, Kazunori, MANALO, Daria Llenaresas, NOGUCHI, Akira, INOUE, Satoshi, PARK, Chun-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305517/
https://www.ncbi.nlm.nih.gov/pubmed/30333382
http://dx.doi.org/10.1292/jvms.18-0519
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author SHIWA, Nozomi
NAKAJIMA, Chikage
KIMITSUKI, Kazunori
MANALO, Daria Llenaresas
NOGUCHI, Akira
INOUE, Satoshi
PARK, Chun-Ho
author_facet SHIWA, Nozomi
NAKAJIMA, Chikage
KIMITSUKI, Kazunori
MANALO, Daria Llenaresas
NOGUCHI, Akira
INOUE, Satoshi
PARK, Chun-Ho
author_sort SHIWA, Nozomi
collection PubMed
description Recently, we reported that follicle-sinus complexes (FSCs) in the muzzle skin are useful for postmortem diagnosis of rabid dogs. Here, we compared the sensitivity and specificity of detecting the viral antigen in the brain and FSCs of 226 suspected rabid dogs, and assessed whether the FSC harbored the virus genome and particles. The viral antigen was detected in 211 of 226 samples with 100% sensitivity and specificity. Viral RNA and particles were observed in the cytoplasm of Merkel cells (MCs). These results suggest that MCs are targets of virus infection and FSCs are useful material for diagnosing rabies.
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spelling pubmed-63055172018-12-28 Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs SHIWA, Nozomi NAKAJIMA, Chikage KIMITSUKI, Kazunori MANALO, Daria Llenaresas NOGUCHI, Akira INOUE, Satoshi PARK, Chun-Ho J Vet Med Sci Pathology Recently, we reported that follicle-sinus complexes (FSCs) in the muzzle skin are useful for postmortem diagnosis of rabid dogs. Here, we compared the sensitivity and specificity of detecting the viral antigen in the brain and FSCs of 226 suspected rabid dogs, and assessed whether the FSC harbored the virus genome and particles. The viral antigen was detected in 211 of 226 samples with 100% sensitivity and specificity. Viral RNA and particles were observed in the cytoplasm of Merkel cells (MCs). These results suggest that MCs are targets of virus infection and FSCs are useful material for diagnosing rabies. The Japanese Society of Veterinary Science 2018-10-17 2018-12 /pmc/articles/PMC6305517/ /pubmed/30333382 http://dx.doi.org/10.1292/jvms.18-0519 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Pathology
SHIWA, Nozomi
NAKAJIMA, Chikage
KIMITSUKI, Kazunori
MANALO, Daria Llenaresas
NOGUCHI, Akira
INOUE, Satoshi
PARK, Chun-Ho
Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs
title Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs
title_full Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs
title_fullStr Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs
title_full_unstemmed Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs
title_short Follicle sinus complexes (FSCs) in muzzle skin as postmortem diagnostic material of rabid dogs
title_sort follicle sinus complexes (fscs) in muzzle skin as postmortem diagnostic material of rabid dogs
topic Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305517/
https://www.ncbi.nlm.nih.gov/pubmed/30333382
http://dx.doi.org/10.1292/jvms.18-0519
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