Cargando…
miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2
MicroRNAs (miRNAs), a class of small non-coding RNAs, have been proved as novel and potent regulators of adipogenesis. A previous study has found out that miR-144-3p was a biomarker of type 2 diabetes, but the role of miR-144-3p in regulating adipogenesis was still unclear. In the present study, the...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305703/ https://www.ncbi.nlm.nih.gov/pubmed/30619490 http://dx.doi.org/10.3389/fgene.2018.00677 |
_version_ | 1783382626250260480 |
---|---|
author | Shen, Linyuan Li, Qiang Wang, Jinyong Zhao, Ye Niu, Lili Bai, Lin Shuai, Surong Li, Xuewei Zhang, Shunhua Zhu, Li |
author_facet | Shen, Linyuan Li, Qiang Wang, Jinyong Zhao, Ye Niu, Lili Bai, Lin Shuai, Surong Li, Xuewei Zhang, Shunhua Zhu, Li |
author_sort | Shen, Linyuan |
collection | PubMed |
description | MicroRNAs (miRNAs), a class of small non-coding RNAs, have been proved as novel and potent regulators of adipogenesis. A previous study has found out that miR-144-3p was a biomarker of type 2 diabetes, but the role of miR-144-3p in regulating adipogenesis was still unclear. In the present study, the expression of miR-144-3p increased in obese mice and during the 3T3-L1 differentiation process. Overexpression of miR-144-3p suppressed the expression of cell cycle regulatory factors and inhibited pre-adipocytes proliferation. Besides, overexpression of miR-144-3p accelerated lipid accumulation in adipocytes and positively regulated adipogenesis, which was also accompanied by increasing the expression of genes related to fatty acid synthesis and decreasing the expression of genes involved in fatty acid oxidation. Furthermore, luciferase activity assays indicated that miR-144-3p directly targeted Klf3 and CtBP2. The process was also confirmed by the mRNA and protein expression of Klf3 and CtBP2, which were suppressed by miR-144-3p. Furthermore, miR-144-3p targeting Klf3/CtBP2 would induce C/EBPα activity by releasing corepressors (Klf3 and CtBP2) from its promoter region. Moreover, we also observed that miR-144-3p could promote adipogenesis in mice injected with miR-144-3p agomir through tail-vein injection. Taken together, these results support that miR-144-3p can facilitate adipogenesis both in vitro and in vivo, which implies that miR-144-3p could be a target for therapeutic intervention in obesity and metabolic syndrome in the future. |
format | Online Article Text |
id | pubmed-6305703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63057032019-01-07 miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2 Shen, Linyuan Li, Qiang Wang, Jinyong Zhao, Ye Niu, Lili Bai, Lin Shuai, Surong Li, Xuewei Zhang, Shunhua Zhu, Li Front Genet Genetics MicroRNAs (miRNAs), a class of small non-coding RNAs, have been proved as novel and potent regulators of adipogenesis. A previous study has found out that miR-144-3p was a biomarker of type 2 diabetes, but the role of miR-144-3p in regulating adipogenesis was still unclear. In the present study, the expression of miR-144-3p increased in obese mice and during the 3T3-L1 differentiation process. Overexpression of miR-144-3p suppressed the expression of cell cycle regulatory factors and inhibited pre-adipocytes proliferation. Besides, overexpression of miR-144-3p accelerated lipid accumulation in adipocytes and positively regulated adipogenesis, which was also accompanied by increasing the expression of genes related to fatty acid synthesis and decreasing the expression of genes involved in fatty acid oxidation. Furthermore, luciferase activity assays indicated that miR-144-3p directly targeted Klf3 and CtBP2. The process was also confirmed by the mRNA and protein expression of Klf3 and CtBP2, which were suppressed by miR-144-3p. Furthermore, miR-144-3p targeting Klf3/CtBP2 would induce C/EBPα activity by releasing corepressors (Klf3 and CtBP2) from its promoter region. Moreover, we also observed that miR-144-3p could promote adipogenesis in mice injected with miR-144-3p agomir through tail-vein injection. Taken together, these results support that miR-144-3p can facilitate adipogenesis both in vitro and in vivo, which implies that miR-144-3p could be a target for therapeutic intervention in obesity and metabolic syndrome in the future. Frontiers Media S.A. 2018-12-19 /pmc/articles/PMC6305703/ /pubmed/30619490 http://dx.doi.org/10.3389/fgene.2018.00677 Text en Copyright © 2018 Shen, Li, Wang, Zhao, Niu, Bai, Shuai, Li, Zhang and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Shen, Linyuan Li, Qiang Wang, Jinyong Zhao, Ye Niu, Lili Bai, Lin Shuai, Surong Li, Xuewei Zhang, Shunhua Zhu, Li miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2 |
title | miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2 |
title_full | miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2 |
title_fullStr | miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2 |
title_full_unstemmed | miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2 |
title_short | miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2 |
title_sort | mir-144-3p promotes adipogenesis through releasing c/ebpα from klf3 and ctbp2 |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305703/ https://www.ncbi.nlm.nih.gov/pubmed/30619490 http://dx.doi.org/10.3389/fgene.2018.00677 |
work_keys_str_mv | AT shenlinyuan mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT liqiang mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT wangjinyong mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT zhaoye mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT niulili mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT bailin mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT shuaisurong mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT lixuewei mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT zhangshunhua mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 AT zhuli mir1443ppromotesadipogenesisthroughreleasingcebpafromklf3andctbp2 |