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Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells
BACKGROUND: Targeted co-delivery of siRNA and a chemotherapeutic drug is an attractive approach to cancer drug design and treatment. This study was carried out to design an anti-Mucin1 aptamer (Apt)-conjugated chitosan nanoparticle (NP) for targeted co-delivery of insulin-like growth factor receptor...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pasteur Institute
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305820/ https://www.ncbi.nlm.nih.gov/pubmed/30041514 http://dx.doi.org/10.29252/.23.1.21 |
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author | Jafari, Reza Zolbanin, Naime Majidi Majidi, Jafar Atyabi, Fatemeh Yousefi, Mehdi Jadidi-Niaragh, Farhad Aghebati-Maleki, Leili Shanehbandi, Dariush Zangbar, Mohammad-Sadegh Soltani Rafatpanah, Houshang |
author_facet | Jafari, Reza Zolbanin, Naime Majidi Majidi, Jafar Atyabi, Fatemeh Yousefi, Mehdi Jadidi-Niaragh, Farhad Aghebati-Maleki, Leili Shanehbandi, Dariush Zangbar, Mohammad-Sadegh Soltani Rafatpanah, Houshang |
author_sort | Jafari, Reza |
collection | PubMed |
description | BACKGROUND: Targeted co-delivery of siRNA and a chemotherapeutic drug is an attractive approach to cancer drug design and treatment. This study was carried out to design an anti-Mucin1 aptamer (Apt)-conjugated chitosan nanoparticle (NP) for targeted co-delivery of insulin-like growth factor receptor 1 (IGF-1R) Silencer siRNA and docetaxel (DTX) to SKBR3 cells. METHODS: Characterization of nano-drugs, cellular uptake of NPs, cell viability, and gene expression studies were evaluated based on metastatic breast cancer cells. RESULTS: The results of this study showed that NPs had spherical and smooth morphology with 110-118 nm in size and had positive zeta potential (12-14 mV). siRNA and DTX were considerably loaded into NPs. The appropriate conjugation of the Apt to the NPs was affirmed by gel electrophoresis. The Apt-conjugated NPs were observed to enhance the cellular uptake of NPs into the SKBR3 cells. Although the combination treatment significantly decreased the cell viability of SKBR3 cells, the augmentative effect was observed when Apt was conjugated to NPs. Furthermore, Apt-conjugated NPs dramatically reduced the genetic expression of IGF-1R, signal transducers and activators of transcription 3 (STAT3), matrix metalloproteinases (MMP9), and vascular growth factor (VEGF). CONCLUSION: The targeted NPs may augment the targeting of pathways involved in tumorigenesis and metastasis of breast cancer. Therefore, more animal model experiments are needed to further clarify the efficacy and safety of this functionalized nanodrug. |
format | Online Article Text |
id | pubmed-6305820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Pasteur Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-63058202019-01-10 Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells Jafari, Reza Zolbanin, Naime Majidi Majidi, Jafar Atyabi, Fatemeh Yousefi, Mehdi Jadidi-Niaragh, Farhad Aghebati-Maleki, Leili Shanehbandi, Dariush Zangbar, Mohammad-Sadegh Soltani Rafatpanah, Houshang Iran Biomed J Full Length BACKGROUND: Targeted co-delivery of siRNA and a chemotherapeutic drug is an attractive approach to cancer drug design and treatment. This study was carried out to design an anti-Mucin1 aptamer (Apt)-conjugated chitosan nanoparticle (NP) for targeted co-delivery of insulin-like growth factor receptor 1 (IGF-1R) Silencer siRNA and docetaxel (DTX) to SKBR3 cells. METHODS: Characterization of nano-drugs, cellular uptake of NPs, cell viability, and gene expression studies were evaluated based on metastatic breast cancer cells. RESULTS: The results of this study showed that NPs had spherical and smooth morphology with 110-118 nm in size and had positive zeta potential (12-14 mV). siRNA and DTX were considerably loaded into NPs. The appropriate conjugation of the Apt to the NPs was affirmed by gel electrophoresis. The Apt-conjugated NPs were observed to enhance the cellular uptake of NPs into the SKBR3 cells. Although the combination treatment significantly decreased the cell viability of SKBR3 cells, the augmentative effect was observed when Apt was conjugated to NPs. Furthermore, Apt-conjugated NPs dramatically reduced the genetic expression of IGF-1R, signal transducers and activators of transcription 3 (STAT3), matrix metalloproteinases (MMP9), and vascular growth factor (VEGF). CONCLUSION: The targeted NPs may augment the targeting of pathways involved in tumorigenesis and metastasis of breast cancer. Therefore, more animal model experiments are needed to further clarify the efficacy and safety of this functionalized nanodrug. Pasteur Institute 2019-01 /pmc/articles/PMC6305820/ /pubmed/30041514 http://dx.doi.org/10.29252/.23.1.21 Text en Copyright: © Iranian Biomedical Journal http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Length Jafari, Reza Zolbanin, Naime Majidi Majidi, Jafar Atyabi, Fatemeh Yousefi, Mehdi Jadidi-Niaragh, Farhad Aghebati-Maleki, Leili Shanehbandi, Dariush Zangbar, Mohammad-Sadegh Soltani Rafatpanah, Houshang Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells |
title | Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells |
title_full | Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells |
title_fullStr | Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells |
title_full_unstemmed | Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells |
title_short | Anti-Mucin1 Aptamer-Conjugated Chitosan Nanoparticles for Targeted Co-Delivery of Docetaxel and IGF-1R siRNA to SKBR3 Metastatic Breast Cancer Cells |
title_sort | anti-mucin1 aptamer-conjugated chitosan nanoparticles for targeted co-delivery of docetaxel and igf-1r sirna to skbr3 metastatic breast cancer cells |
topic | Full Length |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305820/ https://www.ncbi.nlm.nih.gov/pubmed/30041514 http://dx.doi.org/10.29252/.23.1.21 |
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