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Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress

Laminarin (LAM) is a β-glucan oligomer known to possess biological activities such as anticancer and antioxidant effects. This study explored the influence of LAM supplementation on in vitro aged porcine oocytes and the underlying mechanisms behind this influence. We found that LAM delayed the aging...

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Autores principales: YAO, Xuerui, JIANG, Hao, LIANG, Shuang, SHEN, Xinghui, GAO, Qingshan, XU, Yong Nan, KIM, Nam-Hyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305855/
https://www.ncbi.nlm.nih.gov/pubmed/30270255
http://dx.doi.org/10.1262/jrd.2018-031
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author YAO, Xuerui
JIANG, Hao
LIANG, Shuang
SHEN, Xinghui
GAO, Qingshan
XU, Yong Nan
KIM, Nam-Hyung
author_facet YAO, Xuerui
JIANG, Hao
LIANG, Shuang
SHEN, Xinghui
GAO, Qingshan
XU, Yong Nan
KIM, Nam-Hyung
author_sort YAO, Xuerui
collection PubMed
description Laminarin (LAM) is a β-glucan oligomer known to possess biological activities such as anticancer and antioxidant effects. This study explored the influence of LAM supplementation on in vitro aged porcine oocytes and the underlying mechanisms behind this influence. We found that LAM delayed the aging process and improved the quality of aged oocytes. LAM supplementation enhanced the subsequent developmental competence of aged oocytes during the in vitro aging process. The blastocyst formation rate was significantly increased in aged oocytes treated with 20 µg/ml LAM compared to non-treated aged oocytes (45.3% vs. 28.7%, P < 0.01). The mRNA levels of apoptosis-related genes, B cell lymphoma-2-associated X protein (Bax) and Caspase-3, were significantly lower in blastocysts derived from the LAM-treated aged oocytes during the in vitro aging process. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased in aged oocytes following LAM treatment. Mitochondrial membrane potential was increased, and the activities of caspase-3 and cathepsin B were significantly reduced in the LAM-treated aged oocytes compared with the non-treated aged oocytes. Taken together, these results suggest that LAM is beneficial for delaying the aging process in porcine oocytes.
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spelling pubmed-63058552019-01-03 Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress YAO, Xuerui JIANG, Hao LIANG, Shuang SHEN, Xinghui GAO, Qingshan XU, Yong Nan KIM, Nam-Hyung J Reprod Dev Original Article Laminarin (LAM) is a β-glucan oligomer known to possess biological activities such as anticancer and antioxidant effects. This study explored the influence of LAM supplementation on in vitro aged porcine oocytes and the underlying mechanisms behind this influence. We found that LAM delayed the aging process and improved the quality of aged oocytes. LAM supplementation enhanced the subsequent developmental competence of aged oocytes during the in vitro aging process. The blastocyst formation rate was significantly increased in aged oocytes treated with 20 µg/ml LAM compared to non-treated aged oocytes (45.3% vs. 28.7%, P < 0.01). The mRNA levels of apoptosis-related genes, B cell lymphoma-2-associated X protein (Bax) and Caspase-3, were significantly lower in blastocysts derived from the LAM-treated aged oocytes during the in vitro aging process. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased in aged oocytes following LAM treatment. Mitochondrial membrane potential was increased, and the activities of caspase-3 and cathepsin B were significantly reduced in the LAM-treated aged oocytes compared with the non-treated aged oocytes. Taken together, these results suggest that LAM is beneficial for delaying the aging process in porcine oocytes. The Society for Reproduction and Development 2018-10-01 2018-12 /pmc/articles/PMC6305855/ /pubmed/30270255 http://dx.doi.org/10.1262/jrd.2018-031 Text en ©2018 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
YAO, Xuerui
JIANG, Hao
LIANG, Shuang
SHEN, Xinghui
GAO, Qingshan
XU, Yong Nan
KIM, Nam-Hyung
Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
title Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
title_full Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
title_fullStr Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
title_full_unstemmed Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
title_short Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
title_sort laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305855/
https://www.ncbi.nlm.nih.gov/pubmed/30270255
http://dx.doi.org/10.1262/jrd.2018-031
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